Palobart G, Juárez A, Tomas J, Regué M
Departamento de Microbiología y Parasitología Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Spain.
Microbios. 1991;67(272-273):171-6.
Serratia marcescens 2170 derivative strains harbouring either the haemolytic plasmid pANN202-312 or pANN202-312R, were able to produce extracellular haemolysin to a greater extent than the corresponding Escherichia coli 5K strains. The haemolysin recovered from S. marcescens cultures supernatants had a molecular weight of 110 kD, and its extracellular production was maximal near the end of the exponential growth phase. The hlyR sequence, which belongs to the haemolytic determinant located in the plasmid pHly152 and enhances haemolysin expression and secretion in E. coli, appeared not to play an equivalent role in S. marcescens.
携带溶血性质粒pANN202 - 312或pANN202 - 312R的粘质沙雷氏菌2170衍生菌株，比相应的大肠杆菌5K菌株能够在更大程度上产生细胞外溶血素。从粘质沙雷氏菌培养上清液中回收的溶血素分子量为110 kD，其细胞外产生在指数生长期接近结束时达到最大。hlyR序列属于位于质粒pHly152中的溶血决定簇，可增强大肠杆菌中溶血素的表达和分泌，但在粘质沙雷氏菌中似乎不发挥同等作用。
