Chen Hao Dong, Ge Kui Kui, Li Yin Mei, Wu Jian Guang, Gu Yong Qiang, Wei Hai Ming, Tian Zhi Gang
Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Cell Mol Immunol. 2007 Jun;4(3):221-5.
Lymphocyte function associated antigen-1 (CD11a/CD18, LFA-1) plays an important role in the structure of the immunological synapse and is required for efficient lysis of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. To study the activation mode of LFA-1 on the NK cell surface, optical tweezers were used in the work. As an emerging technology, optical tweezers are widely used to manipulate microscopic objects and measure the forces of molecular interactions in the field of biological research. In our study, a new platform was constructed to study the single molecular behavior of receptor on cell surface using optical tweezers. Based on the platform, the interaction between an NK cell and a polystyrene microsphere coated with anti-LFA-1 antibody was observed. The result confirmed that the adhesion forces between an NK cell and a polystyrene bead were time-dependent. According to our findings, we propose that anti-LFA-1 antibody may cause the clustering of LFA-1 on NK cell surface.
淋巴细胞功能相关抗原-1（CD11a/CD18，LFA-1）在免疫突触结构中起重要作用，是细胞毒性T淋巴细胞（CTL）和自然杀伤（NK）细胞有效杀伤所必需的。为了研究LFA-1在NK细胞表面的激活模式，该研究使用了光镊。作为一项新兴技术，光镊在生物研究领域被广泛用于操纵微观物体和测量分子相互作用的力。在我们的研究中，构建了一个新平台，用于使用光镊研究细胞表面受体的单分子行为。基于该平台，观察了NK细胞与包被抗LFA-1抗体的聚苯乙烯微球之间的相互作用。结果证实，NK细胞与聚苯乙烯珠之间的粘附力是时间依赖性的。根据我们的发现，我们提出抗LFA-1抗体可能导致LFA-1在NK细胞表面聚集。
