López-Labrador F Xavier, Dove Lorna, Hui Chee-Kin, Phung Yume, Kim Michael, Berenguer Marina, Wright Teresa L
Public Health Department, Generalitat Valenciana and Evolutionary Genetics Unit, Institut Cavanilles de Biodiversitat i Biologia Evolutiva, University of Valencia, Apt. Of. 2085, E-46071 Valencia, Spain.
Virus Res. 2007 Dec;130(1-2):285-91. doi: 10.1016/j.virusres.2007.05.016. Epub 2007 Jun 29.
Chronic infection by Hepatitis C Virus (HCV) causes liver fibrosis, which is accelerated by unknown mechanisms in patients with HIV-1 coinfection. The evolution of HCV quasispecies in this setting of coinfection is not fully understood. To compare HCV quasispecies between HIV-HCV coinfection and HCV monoinfection, we sequenced 340 HCV clones from the HVR-1 and NS3 regions at two different time points in two groups of treatment-naïve patients with HCV-1a infection: (1) HIV-HCV positive (n=6); and (2) HIV negative-HCV positive (n=3). In HCV/HIV coinfection, we found a trend for reduced HCV genetic complexity and diversity, and a trend towards reduced dN/dS ratios in the HVR-1 region, especially in those patients with CD4<200cells/mm(3), who lost positive selective immune pressure in the HVR-1 region. Differences in immune regulation of HCV quasispecies in HIV coinfected individuals deserve further exploration to clarify the different outcomes of chronic hepatitis C noted between the immunocompromised and the immunocompetent host.
丙型肝炎病毒（HCV）的慢性感染会导致肝纤维化，在合并感染HIV-1的患者中，肝纤维化会通过未知机制加速发展。在这种合并感染情况下，HCV准种的演变尚未完全明确。为了比较HIV-HCV合并感染与HCV单一感染之间的HCV准种，我们在两组未经治疗的HCV-1a感染患者的两个不同时间点，对来自高变区1（HVR-1）和NS3区域的340个HCV克隆进行了测序：（1）HIV-HCV阳性（n = 6）；（2）HIV阴性-HCV阳性（n = 3）。在HCV/HIV合并感染中，我们发现HCV基因复杂性和多样性有降低的趋势，并且在HVR-1区域dN/dS比值有降低的趋势，尤其是在那些CD4<200细胞/mm³的患者中，他们在HVR-1区域失去了阳性选择免疫压力。对于HIV合并感染个体中HCV准种免疫调节的差异，值得进一步探索，以阐明免疫受损宿主和免疫健全宿主之间慢性丙型肝炎的不同结局。
