Wu Jiguo, Lin Li, Luan Tiangang, Chan Gilbert Yuk Sing, Lan Chongyu
State Key Laboratory of Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
Food Chem Toxicol. 2007 Oct;45(10):2057-63. doi: 10.1016/j.fct.2007.05.006. Epub 2007 May 23.
The effects of organophosphorus pesticides (OPs), oxons and their ozonation byproducts on gap junctional intercellular communication (GJIC) on cultured BRL cell line were investigated using scrape loading and dye transfer (SL/DT) technique. The neutral red uptake assay was used to identify the non-cytotoxic levels of diazinon, parathion and methyl-parathion applied to GJIC assay. The concentration-dependent inhibition of GJIC was observed over a range of 50-350 mg/l diazinon, parathion and methyl-parathion after 90 min incubation compared with the vehicle control. However, oxons and ozonation byproducts of OPs had no inhibition effect on GJIC at any of the concentrations tested. The inhibition of GJIC by OPs was reversible after removal of the tested pesticides followed by incubation with fresh medium. The present study suggested that the ozonation treatment could be used for the detoxification of drinking water and food crops contaminated with diazinon, parathion and methyl-parathion without formation of GJIC toxicity.
采用刮除加载和染料转移（SL/DT）技术，研究了有机磷农药（OPs）、氧化磷及其臭氧化副产物对培养的BRL细胞系间隙连接细胞间通讯（GJIC）的影响。采用中性红摄取试验确定用于GJIC试验的二嗪农、对硫磷和甲基对硫磷的无细胞毒性水平。与溶剂对照相比，在孵育90分钟后，在50-350mg/l的二嗪农、对硫磷和甲基对硫磷范围内观察到GJIC的浓度依赖性抑制。然而，氧化磷和OPs的臭氧化副产物在任何测试浓度下均对GJIC无抑制作用。去除受试农药后，再与新鲜培养基孵育，OPs对GJIC的抑制作用是可逆的。本研究表明，臭氧化处理可用于对受二嗪农、对硫磷和甲基对硫磷污染的饮用水和粮食作物进行解毒，而不会形成GJIC毒性。
