利用洋葱根尖细胞微核试验检测蝇毒磷的潜在细胞毒性。
Potential cytotoxic effect of Anilofos by using Allium cepa assay.
机构信息
Department of Biology, Faculty of Arts and Sciences, Afyon Kocatepe University, Afyonkarahisar, Turkey,
出版信息
Cytotechnology. 2015 Oct;67(5):783-91. doi: 10.1007/s10616-014-9716-1. Epub 2014 May 17.
Abstract
Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC50 value was determined 50 ppm and 1/2× EC50 (25 ppm), EC50 (50 ppm) and 2 × EC50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.
摘要
广泛应用于农业的磷胺对洋葱根尖细胞有遗传毒性。在洋葱根尖生长抑制试验中，磷胺的 EC50 值为 50ppm，1/2×EC50（25ppm）、EC50（50ppm）和 2×EC50（100ppm）浓度被应用于洋葱根尖。实验中同时设置了阴性和阳性对照。结果表明，随着磷胺浓度的增加，所有处理组在各暴露时间的有丝分裂指数均降低，同时观察到后期-末期染色体滞后、粘连和后期桥。在后期-末期细胞中，还观察到中期 c-有丝分裂、核紊乱和双核细胞等其他异常。这些结果还通过 Dunnett t 检验（双侧）进行了统计学分析，所有浓度的磷胺均被发现具有显著性。
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