从阿拉伯胶合欢中分离得到的精胺生物碱的抗疟活性。

The antiplasmodial activity of spermine alkaloids isolated from Albizia gummifera.

作者信息

Rukunga G M, Muregi F W, Tolo F M, Omar S A, Mwitari P, Muthaura C N, Omlin F, Lwande W, Hassanali A, Githure J, Iraqi F W, Mungai G M, Kraus W, Kofi-Tsekpo W M

机构信息

Kenya Medical Research Institute, PO BOX 54840, Nairobi, Kenya.

出版信息

Fitoterapia. 2007 Dec;78(7-8):455-9. doi: 10.1016/j.fitote.2007.02.012. Epub 2007 May 10.

DOI:10.1016/j.fitote.2007.02.012

PMID:17601685

Abstract

In the present study the methanolic extract of Albizia gummifera was fractionated into various fractions. These fractions were tested against choroquine sensitive (NF54) and resistant (ENT30) strains of Plasmodium falciparum. All other fractions apart from the alkaloidal fraction showed low activity with IC 50 above 3 microg/ml. The alkaloidal fraction exhibited strong activity against NF54 and ENT30 with IC 50 of 0.16+/-0.05 and 0.99+/-0.06 microg/ml, respectively. Five known spermine alkaloids were isolated from the alkaloidal fraction. These alkaloids exhibited activities against NF54 and ENT30 with IC 50 ranging from 0.09+/-0.02 to 0.91+/-0.10 microg/ml. Four of the alkaloids were further evaluated for in vivo activity against rodent malaria parasite Plasmodium berghei. The alkaloids showed percentage chemosuppression of parasitaemia in mice ranging from 43 to 72%. The use of the extracts A. gummifera for treatment of malaria in traditional medicine seems to have a scientific basis.

摘要

在本研究中，将阿拉伯胶合欢的甲醇提取物分离成不同的馏分。这些馏分针对恶性疟原虫的氯喹敏感株（NF54）和耐药株（ENT30）进行了测试。除生物碱馏分之外的所有其他馏分均显示出低活性，半数抑制浓度（IC50）高于3微克/毫升。生物碱馏分对NF54和ENT30表现出较强活性，IC50分别为0.16±0.05和0.99±0.06微克/毫升。从生物碱馏分中分离出了五种已知的精胺生物碱。这些生物碱对NF54和ENT30表现出活性，IC50范围为0.09±0.02至0.91±0.10微克/毫升。对其中四种生物碱针对啮齿动物疟原虫伯氏疟原虫的体内活性进行了进一步评估。这些生物碱在小鼠体内对疟原虫血症的化学抑制百分比为43%至72%。在传统医学中使用阿拉伯胶合欢提取物治疗疟疾似乎有科学依据。