Sandhya S, Sarayu K, Uma B, Swaminathan K
National Environmental Engineering Research Institute, CSIR-Complex, Chennai 600113, India.
Bioresour Technol. 2008 May;99(7):2187-91. doi: 10.1016/j.biortech.2007.05.027. Epub 2007 Jun 29.
PCR amplified product containing gene responsible for dye decolorization was cloned and expressed in Escherichia coli. The resulting recombinant strain E. coli SS125 decolorized 200mg/l azo dye (Remazol Red) at 30 degrees C at 255 mg cell/l/h, while the host E. coli (DH5 alpha) had no color removal ability. The dependence of the decolorization rate on initial dye concentration and the maximum rate occurred with the dye at 100 mg l(-1). The decolorization rate of E. coli SS125 was optimal at 37-45 degrees C. Aeration strongly-inhibited the decolorization, but decolorization occurred effectively under static and anaerobic incubation conditions. The E. coli SS125 strain also exhibited excellent stability during reported batch operation.
将含有负责染料脱色基因的PCR扩增产物克隆并在大肠杆菌中表达。所得重组菌株大肠杆菌SS125在30℃下以255mg细胞/升/小时的速度使200mg/升偶氮染料（雷马素红）脱色，而宿主大肠杆菌（DH5α）没有脱色能力。脱色率对初始染料浓度的依赖性以及最大速率出现在染料浓度为100mg l(-1)时。大肠杆菌SS125的脱色率在37 - 45℃时最佳。通气强烈抑制脱色，但在静态和厌氧培养条件下脱色有效。大肠杆菌SS125菌株在报告的分批操作过程中也表现出优异的稳定性。
