Liu Guangfei, Zhou Jiti, Wang Jing, Zhou Mi, Lu Hong, Jin Ruofei
Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China.
Bioresour Technol. 2009 Jun;100(11):2791-5. doi: 10.1016/j.biortech.2008.12.040. Epub 2009 Feb 8.
This study demonstrated the effective application of intracellular azoreductase in mediated decolorization of azo dyes. Using the quinone reductase activity of overexpressed azoreductase AZR and quinone redox mediators, the decolorization performance of the recombinant strain Escherichia coli YB was significantly enhanced. In the presence of 0.2 mM lawsone, 75% acid red 27 (1 mM) was decolorized by E. coli YB in only 2 h, which was the highest bacterial decolorization rate ever reported. Compared to lawsone, menadione was a less effective redox mediator. Glucose was found to be the best carbon source for mediated decolorization by E. coli YB. The recombinant strain could complete four rounds of mediated decolorization repeatedly in 12 h. In addition, a 10-min pre-incubation of E. coli JM109 and activated sludge with 2-methylhydroquinone resulted in great improvement of mediated decolorization performance, which may be applied in practical treatment.
本研究证明了细胞内偶氮还原酶在介导偶氮染料脱色中的有效应用。利用过表达的偶氮还原酶AZR的醌还原酶活性和醌氧化还原介质，重组菌株大肠杆菌YB的脱色性能得到显著提高。在存在0.2 mM胡桃醌的情况下，大肠杆菌YB仅在2小时内就使75%的酸性红27（1 mM）脱色，这是有史以来报道的最高细菌脱色率。与胡桃醌相比，甲萘醌是一种效果较差的氧化还原介质。发现葡萄糖是大肠杆菌YB介导脱色的最佳碳源。该重组菌株可在12小时内重复完成四轮介导脱色。此外，大肠杆菌JM109和活性污泥与2-甲基对苯二酚预孵育10分钟可使介导脱色性能得到极大改善，这可能应用于实际处理。
