Rucinska Agata, Kirko Sergej, Gabryelak Teresa
Department of General Biophysics, University of Lodz, Banacha 12/16, Lodz 90-237, Poland.
Cell Biol Int. 2007 Nov;31(11):1371-8. doi: 10.1016/j.cellbi.2007.05.012. Epub 2007 May 21.
Genistein-8-C-glucoside (G8CG) belongs to isoflavones, which are a subclass of flavonoids, a large group of polyphenolic compounds widely distributed in plants. A number of studies on flavonoids show their cardioprotective and antiosteoporosis properties in in vitro and in vivo models. As a phytoestrogen, genistein has recently generated interest as a potential anticancer and antiatherogenic agent. Several flavonoids are known as antioxidants and scavengers of free oxygen radicals. In the current investigation we used glycosylated genistein (genistein-8-C-glucoside) from flowers of lupine (Lupinus luteus L.). Many authors have found that the action of genistein is not so simple, although many reports conducted in vitro have demonstrated that it is cytotoxic and genotoxic. Therefore, the cytotoxic and genotoxic effects of this compound in Chinese hamster ovary cells (line CHO) were studied. A colorimetric MTT assay to assess cytotoxicity and a Comet assay for the detection of DNA damage were used. Apoptosis was determined by the Hoechst 33258/propidium iodide staining technique. We have also demonstrated antioxidant properties of G8CG. The level of reactive oxygen species generated by G8CG alone and/or H2O2 was evaluated with fluorescence probes: dichlorofluorescein-diacetate (DCFDA) by flow cytometry. The cells were exposed to various concentrations of genistein-8-C-glucoside (1-290 microM) and hydrogen peroxide (10-130 microM) and the effect of G8CG alone or in combination with H2O2 was determined. The results reveal that G8CG at concentrations higher than 10 microM significantly reduced cell viability, induced apoptosis and DNA damage. However at lower concentrations (5 and 7.5 microM), G8CG showed antioxidant properties, but had no cytotoxic or genotoxic activity.
染料木黄酮 - 8 - C - 葡萄糖苷（G8CG）属于异黄酮类，而异黄酮是黄酮类化合物的一个亚类，黄酮类是一大类广泛分布于植物中的多酚类化合物。多项关于黄酮类化合物的研究表明，它们在体外和体内模型中具有心脏保护和抗骨质疏松特性。作为一种植物雌激素，染料木黄酮最近作为一种潜在的抗癌和抗动脉粥样硬化剂引起了人们的关注。几种黄酮类化合物被认为是抗氧化剂和游离氧自由基清除剂。在当前的研究中，我们使用了来自羽扇豆（Lupinus luteus L.）花中的糖基化染料木黄酮（染料木黄酮 - 8 - C - 葡萄糖苷）。许多作者发现，染料木黄酮的作用并非如此简单，尽管许多体外研究报告表明它具有细胞毒性和基因毒性。因此，研究了该化合物在中国仓鼠卵巢细胞（CHO细胞系）中的细胞毒性和基因毒性作用。使用比色法MTT试验评估细胞毒性，使用彗星试验检测DNA损伤。通过Hoechst 33258/碘化丙啶染色技术确定细胞凋亡。我们还证明了G8CG的抗氧化特性。使用荧光探针二氯荧光素二乙酸酯（DCFDA）通过流式细胞术评估单独由G8CG和/或过氧化氢产生的活性氧水平。细胞暴露于不同浓度的染料木黄酮 - 8 - C - 葡萄糖苷（1 - 290微摩尔）和过氧化氢（10 - 130微摩尔），并确定单独的G8CG或与过氧化氢联合使用的效果。结果表明，浓度高于10微摩尔的G8CG显著降低细胞活力，诱导细胞凋亡和DNA损伤。然而，在较低浓度（5和7.5微摩尔）下，G8CG表现出抗氧化特性，但没有细胞毒性或基因毒性活性。
