Rucinska Agata, Gabryelak Teresa
Department of General Biophysics, University of Lodz, Banacha 12/16, Lodz 90-237, Poland.
Cell Biol Int. 2009 Feb;33(2):247-52. doi: 10.1016/j.cellbi.2008.11.003. Epub 2008 Nov 21.
Genistein-8-C-glucoside (G8CG) belongs to natural isoflavones phytoestrogens, which are a subclass of flavonoids, a large group of polyphenolic compounds widely distributed in plants, with possible anticarcinogenic effects in various in vitro systems and in vivo animal models. We used glycosylated genistein (genistein-8-C-glucoside) from flowers of lupine (Lupinus luteus L.) to study its cytotoxic and genotoxic effects on mouse embryonic fibroblast (line NIH 3T3). The MTT assay to assess cytotoxicity and comet assay for the detection of DNA damage were used. The cells were exposed to various concentrations of genistein-8-C-glucoside (2.5-110 microM) and hydrogen peroxide (5-90 microM). The effect of G8CG alone or in combination with H2O2 was determined. G8CG at concentrations > 20 microM significantly reduced cell viability and induced DNA damage. In contrast, lower concentrations of (2.5-10 microM) G8CG showed antioxidant properties against H2O2-induced DNA damage with no associated toxicity.
染料木黄酮 -8-C-葡萄糖苷（G8CG）属于天然异黄酮类植物雌激素，是黄酮类化合物的一个亚类，黄酮类是一大类广泛分布于植物中的多酚类化合物，在各种体外系统和体内动物模型中可能具有抗癌作用。我们使用从羽扇豆（Lupinus luteus L.）花中提取的糖基化染料木黄酮（染料木黄酮 -8-C-葡萄糖苷）来研究其对小鼠胚胎成纤维细胞（NIH 3T3细胞系）的细胞毒性和遗传毒性作用。采用MTT法评估细胞毒性，彗星试验检测DNA损伤。将细胞暴露于不同浓度的染料木黄酮 -8-C-葡萄糖苷（2.5 - 110微摩尔）和过氧化氢（5 - 90微摩尔）中。测定了G8CG单独作用或与H2O2联合作用的效果。浓度大于20微摩尔的G8CG显著降低细胞活力并诱导DNA损伤。相比之下，较低浓度（2.5 - 10微摩尔）的G8CG对H2O2诱导的DNA损伤具有抗氧化特性且无相关毒性。
