[玉米黑粉菌线粒体DNA的研究。I. 克隆与基因图谱分析]

[Studies of mtDNA of Ustilago maydis. I. Cloning and gene mapping].

作者信息

Feng G H, Cheng W, Lu S Y

机构信息

Institute of Microbiology, Academia Sinica, Beijing.

出版信息

Yi Chuan Xue Bao. 1991;18(4):378-84.

PMID:1760197

Abstract

This paper covers the following studies of mtDNA of Ustilago maydis. (1) By inserting the Bam HI and Pst I fragments of the mtDNA into the corresponding sites of pBR322, we cloned a unique sequence of 49.6 kb, accounting for 89.3% of the mitochondrial genome (60.7 kb). (2) With heterogenous genes from plants or fungi as probes, we identified seven genes, and mapped them onto the restriction map of the mt DNA. The genes were arranged in such an order: -UmCOB-UmOXII-S-rR NA-UmOXIII-L-rRNA-UmATPase6-UmOXI-. (3) We tried to express the three cloned genes, UmOXII, UmOXIII, and Um-ATPase 6, in E. coli maxcel expression system, but no specific protein was observed.

摘要

本文涵盖了以下对玉米黑粉菌线粒体DNA（mtDNA）的研究。（1）通过将mtDNA的Bam HI和Pst I片段插入pBR322的相应位点，我们克隆了一个49.6 kb的独特序列，占线粒体基因组（60.7 kb）的89.3%。（2）以植物或真菌的异源基因为探针，我们鉴定出了七个基因，并将它们定位到mt DNA的限制酶切图谱上。这些基因按以下顺序排列：-UmCOB-UmOXII-S-rRNA-UmOXIII-L-rRNA-UmATPase6-UmOXI-。（3）我们试图在大肠杆菌maxcel表达系统中表达三个克隆基因，即UmOXII、UmOXIII和Um-ATPase 6，但未观察到特异性蛋白质。