Mira-Moser F, Schofield J C, Orci L
Eur J Clin Invest. 1976 Jan 30;6(1):103-11. doi: 10.1111/j.1365-2362.1976.tb00499.x.
The ionophore A23187 increased the release of rat growth hormone in the presence of a phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine; a second ionophore X537A inhibited growth hormone release induced by the methylxanthine. A23187 did not alter rat growth hormone release in the absence of 3-isobutyl-1-methylxanthine, but X537A enhanced hormone release in the absence of calcium or in the presence or somatostatin. These findings provide further evidence that both calcium and cyclic AMP are important in the regulation of growth hormone release. Tissue incubated in X537A combined electronlucent vesicles apparently derived from the Golgi apparatus, swollen granules and mitochondria with dense matrices. Tissue incubated in the presence of valinomycin or A23187 did not show altered morphology of either secretory granules or the Golgi complex. Possible mechanisms of these changes are discussed.
在磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤存在的情况下，离子载体A23187可增加大鼠生长激素的释放；另一种离子载体X537A可抑制甲基黄嘌呤诱导的生长激素释放。在不存在3-异丁基-1-甲基黄嘌呤的情况下，A23187不会改变大鼠生长激素的释放，但X537A在无钙或存在生长抑素的情况下会增强激素释放。这些发现进一步证明钙和环磷酸腺苷在生长激素释放的调节中都很重要。在X537A中孵育的组织出现明显源自高尔基体的电子透亮小泡、肿胀的颗粒和具有致密基质的线粒体。在缬氨霉素或A23187存在的情况下孵育的组织，其分泌颗粒或高尔基体复合体的形态均未改变。文中讨论了这些变化可能的机制。
