Vetrova E, Esimbekova E, Remmel N, Kotova S, Beloskov N, Kratasyuk V, Gitelson I
Institute of Biophysics, Akademgorodok, 660036 Krasnoyarsk, Russia.
Luminescence. 2007 May-Jun;22(3):206-14. doi: 10.1002/bio.951.
Prototype technologies of a bioluminescent signal system (BSS) based on the luminous bacterium Photobacterium phosphoreum and three enzymatic bioluminescence systems have been proposed for detecting and signalling the presence of toxicants in water systems. A number of pesticides, mostly known as poisonous substances, similar in their structures and physicochemical properties, have been taken as model compounds of chemical agents. The effect of toxicants (organophosphates, derivatives of dithiocarbamide acid, and pyrethroid preparations) on the bioluminescence of the four systems has been analysed. EC(50) and EC(80) have been determined and compared to the maximum permissible concentration for each of the analysed substances. The triple-enzyme systems with ADH and trypsin have been shown to be more sensitive to organophosphorous compounds (0.13-11 mg/L), while the triple-enzyme system with trypsin is highly sensitive to lipotropic poison, a derivative of dithiocarbamine acid (0.03 mg/L). Sensitivities of the triple-enzyme systems to pyrethroid preparations are similar to those of luminous bacteria (0.9-5 mg/L). The results can be used to construct an alarm-test bioluminescence system for detecting chemical toxicants, based on intact bacteria or enzyme systems.
基于发光细菌费氏弧菌的生物发光信号系统（BSS）以及三种酶促生物发光系统的原型技术已被提出，用于检测和指示水系统中有毒物质的存在。一些主要作为有毒物质为人所知、结构和物理化学性质相似的农药，已被用作化学制剂的模型化合物。分析了有毒物质（有机磷酸盐、二硫代氨基甲酸衍生物和拟除虫菊酯制剂）对这四种系统生物发光的影响。已确定了半数有效浓度（EC(50)）和80%有效浓度（EC(80)），并与每种分析物质的最大允许浓度进行了比较。已证明含有乙醇脱氢酶（ADH）和胰蛋白酶的三酶系统对有机磷化合物（0.13 - 11毫克/升）更敏感，而含有胰蛋白酶的三酶系统对亲脂性毒物（一种二硫代氨基甲酸衍生物，0.03毫克/升）高度敏感。三酶系统对拟除虫菊酯制剂的敏感度与发光细菌的敏感度相似（0.9 - 5毫克/升）。这些结果可用于构建基于完整细菌或酶系统的用于检测化学毒物的报警测试生物发光系统。
