Kékedy-Nagy Ladislau, Jun Yao, Darvasi Eugen, Kékedy-Nagy László
Babeş-Bolyai University, Faculty of Chemistry and Chemical Engineering, 400028 Cluj-Napoca, Str. Arany János 11, Romania.
J Biochem Biophys Methods. 2008 Apr 24;70(6):1234-9. doi: 10.1016/j.jbbm.2007.04.006. Epub 2007 May 8.
The zinc content of 3 microL of vegetal samples (tree leaves, lichens and grape sap) atomized from a Pt-wire in the methane-air flame has been determined by atomic absorption spectrometry. The effect of gas flow rates and the atomization height in the flame on the absorption of zinc was evaluated at 213.9 nm. The best results were obtained at a height of 5 mm and gas flow rates of 200 L/h air and 26 L/h methane, respectively. The effect of Na, K, Ca, Mg, SO4(2-), and PO4(3-) on the absorption of zinc was studied too. The detection limit of 0.40+/-0.21 ng was obtained at a significance level of 0.05, using the two-step Neyman-Pearson criterion. The zinc content of the samples has been determined with continuous nebulization and by atomization from the Pt-wire, using both the standard calibration curve and the standard addition method. The results of the two procedures agree within the determination errors.
采用原子吸收光谱法测定了从铂丝雾化进甲烷 - 空气火焰中的3微升植物样品（树叶、地衣和葡萄汁）的锌含量。在213.9纳米处评估了火焰中气体流速和雾化高度对锌吸收的影响。分别在5毫米高度以及空气流速200升/小时和甲烷流速26升/小时的条件下获得了最佳结果。还研究了钠、钾、钙、镁、硫酸根离子和磷酸根离子对锌吸收的影响。使用两步奈曼 - 皮尔逊准则，在0.05的显著性水平下获得了0.40±0.21纳克的检测限。采用连续雾化和从铂丝雾化的方式，使用标准校准曲线和标准加入法测定了样品中的锌含量。两种方法的结果在测定误差范围内一致。
