Pontifícia Universidade Católica do Rio de Janeiro (PUC-Rio), Departamento de Química, Rio de Janeiro, RJ, Brazil.
Talanta. 2010 Oct 15;82(5):1647-53. doi: 10.1016/j.talanta.2010.07.029. Epub 2010 Jul 21.
Cisplatin and carboplatin are the most common platinum-based drugs used in cancer treatment. Pharmacokinetic investigations, the evaluation of the body burden during the treatment, as well as baseline levels of platinum in humans have attracted great interest. Thus, accurate analytical methods for fast and easy Pt monitoring in clinical samples become necessary. In the present study atomic absorption spectrometric methods for the determination of platinum in the forms of cisplatin and carboplatin in human urine were investigated. Platinum, in these different forms, could be determined in urine, after simple sample dilution. Regarding electrothermal atomic absorption spectrometry, the optimum parameters were defined by a central composite design optimization. Multiplicative matrix effects were overcome by using a mixture of HCl and NaCl as modifier. The limit of detection (LOD) was 0.004 mgL(-1) of platinum in the original sample. For the analysis of more concentrated samples, high resolution continuous source flame atomic absorption spectrometry was also investigated. Flame conditions were optimized by a multivariate D-optimal design, using as response the sum of the analyte addition calibration slopes and their standard deviations. Matrix matched external calibration with PtCl(2) calibration solutions, was possible, and the LOD was 0.06 mgL(-1) in the original sample. The results obtained by the proposed procedures were also in good agreement with those obtained by an independent comparative procedure.
顺铂和卡铂是癌症治疗中最常用的两种铂类药物。药代动力学研究、治疗过程中人体负荷的评估以及人类体内铂的基线水平引起了极大的兴趣。因此，需要开发准确、快速、简便的分析方法来监测临床样本中的铂。本研究建立了测定人尿液中顺铂和卡铂的原子吸收光谱法。在简单稀释尿液后，可直接测定这两种不同形式的铂。对于电热原子吸收光谱法，通过中心复合设计优化来确定最佳参数。采用 HCl 和 NaCl 的混合物作为改进剂，克服了乘法基质效应。原始样品中铂的检出限（LOD）为 0.004mg/L。对于更浓缩样品的分析，还研究了高分辨率连续光源火焰原子吸收光谱法。采用多元 D-最优设计，以分析物加标校准斜率及其标准偏差之和作为响应，优化了火焰条件。可以使用 PtCl2 校准溶液进行基体匹配的外部校准，原始样品中的检出限为 0.06mg/L。所提出的程序得到的结果与独立比较程序得到的结果吻合良好。
