van Niftrik Laura, Geerts Willie J C, van Donselaar Elly G, Humbel Bruno M, Yakushevska Alevtyna, Verkleij Arie J, Jetten Mike S M, Strous Marc
Department of Microbiology, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
J Struct Biol. 2008 Mar;161(3):401-10. doi: 10.1016/j.jsb.2007.05.005. Epub 2007 Jun 2.
Anammox bacteria have unique intracellular membranes that divide their cytoplasm into three separate compartments. The largest and innermost cytoplasmic compartment, the anammoxosome, is hypothesized to be the locus of all catabolic reactions in the anammox metabolism. Electron tomography showed that the anammoxosome and its membrane were highly folded. This finding was confirmed by a transmission electron microscopy study using different sample preparation methods. Further, in this study electron-dense particles were observed and electron tomography showed that they were confined to the anammoxosome compartment. Energy dispersive X-ray analysis revealed that these particles contained iron. The functional significance of a highly folded anammoxosome membrane and intracellular iron storage particles are discussed in relation to their possible function in energy generation.
厌氧氨氧化细菌具有独特的内膜，这些内膜将其细胞质分隔成三个独立的区室。最大且最内层的细胞质区室——厌氧氨氧化体，据推测是厌氧氨氧化代谢中所有分解代谢反应的场所。电子断层扫描显示，厌氧氨氧化体及其膜高度折叠。使用不同样品制备方法的透射电子显微镜研究证实了这一发现。此外，在这项研究中观察到了电子致密颗粒，电子断层扫描显示它们局限于厌氧氨氧化体区室。能量色散X射线分析表明这些颗粒含有铁。文中讨论了高度折叠的厌氧氨氧化体膜和细胞内铁储存颗粒在能量产生方面可能发挥的功能及其功能意义。
