矿物质改良剂对荧光假单胞菌对鹰嘴豆枯萎病抑制活性的影响。

Influence of mineral amendment on disease suppressive activity of Pseudomonas fluorescens to Fusarium wilt of chickpea.

作者信息

Saikia Ratul, Varghese Saju, Singh Bhim Pratap, Arora Dilip K

机构信息

National Bureau of Agriculturally Important Microorganisms (NBAIM), Kushmaur, Mau 275 101, Uttar Pradesh, India.

出版信息

Microbiol Res. 2009;164(4):365-73. doi: 10.1016/j.micres.2007.05.001. Epub 2007 Jun 28.

DOI:10.1016/j.micres.2007.05.001

PMID:17604612

Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. ciceri causes considerable yield loss of chickpea. Pseudomonas fluorescens4-92 (Pf4-92) strain can suppress the disease. Amendment of zinc EDTA and copper EDTA could not suppress the disease significantly when used alone; however, they significantly suppressed the disease in presence of Pf4-92. In vitro observation showed that at 40, 30 and 20microgml(-1) concentrations of these minerals, i.e. Zn, Cu and Zn plus Cu, respectively, completely repressed the production of the phytotoxin, fusaric acid (FA). FA concentration (0.5microgml(-1)) has been shown to suppress the production of 2,4-diacetylphloroglucinol (DAPG) by Pf4-92, and DAPG, salicylic acid, pyochelin and pyoluteorin production was enhanced by these mineral amendments. In rockwool bioassays, Zn, Cu and Zn plus Cu amendments reduced FA production and enhanced DAPG production. This study demonstrates that Zn and Cu enhance biocontrol activity by reducing FA produced by the pathogen, F. oxysporum f. sp. ciceri.

摘要

由尖孢镰刀菌鹰嘴豆专化型（Fusarium oxysporum f. sp. ciceri）引起的枯萎病会导致鹰嘴豆产量大幅损失。荧光假单胞菌4-92（Pf4-92）菌株能够抑制该病。单独使用乙二胺四乙酸锌（EDTA锌）和乙二胺四乙酸铜（EDTA铜）时，无法显著抑制该病；然而，在Pf4-92存在的情况下，它们能显著抑制该病。体外观察表明，在这些矿物质（即锌、铜以及锌加铜）浓度分别为40、30和20μg/ml时，可完全抑制植物毒素镰刀菌酸（FA）的产生。已表明FA浓度（0.5μg/ml）会抑制Pf4-92产生2,4-二乙酰基间苯三酚（DAPG），而这些矿物质添加物可增强DAPG、水杨酸、绿脓菌素和藤黄绿菌素的产生。在岩棉生物测定中，锌、铜以及锌加铜的添加物减少了FA的产生并增强了DAPG的产生。本研究表明，锌和铜通过减少病原体尖孢镰刀菌鹰嘴豆专化型产生的FA来增强生物防治活性。