Kankariya Raksha A, Chaudhari Ambalal B, Dandi Navin D
School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, M.S., 425001, India.
Drs. Kiran &, Pallavi Patel Global University (KPGU), Vadodara, Gujarat, India.
Biotechnol Lett. 2025 Jun 17;47(4):64. doi: 10.1007/s10529-025-03606-3.
The fungal contaminated crops or post-harvest products are unfit for consumption, hazardous to human health and threat to food safety. Soil borne phytopathogenic microbes cause severe yield losses at various stages and contribute to 40-50% crop mortality. Post-harvest fungal deterioration during storage is frequently observed primarily because of high oil content. At present, chemical disinfectants and agrichemicals are extensively used to control seed contaminating fungal infection as seed priming agent and/or post-harvest storage protectants. However, application of synthetic fungicides requires experimental evaluation of several parameters and raised serious concerns about emergence of fungicide resistance. On this streak, natural anti-microbial agents such as microbial secondary metabolites are preferred as eco-friendly and cost-effective alternative for seed protection. For this purpose, the use of broad-spectrum antibiotics such as 2,4-diacetylphloroglucinol can warrant potential benefit for seed priming over chemical fungicides. In the present study, it showed maximum inhibitory activity against aflatoxin producing Aspergillus flavus as well as other phytopathogenic fungi viz. Ustilago maydis, Fusarium oxysporum, Sclerotinia sclerotiorum, Colletotrichum circinans and Colletotrichum dematium. It was therefore explored for seed priming of groundnut for repressed proliferation of mycotoxin producing A. flavus. Under pot experiments, 2,4-diacetylphloroglucinol primed seeds showed long-term post-harvest preservation, enhanced seed germination and growth indices as compared to methanol primed and non-primed seeds. 2,4-diacetylphloroglucinol priming is therefore, proposed as a low-cost, dual-benefit seed storage protectant for control of post-harvest fungal deterioration of oilseeds as well as a phytostimulant for enhanced plant growth in field after long-term storage.
被真菌污染的作物或收获后的产品不宜食用，对人体健康有害，且对食品安全构成威胁。土壤传播的植物病原微生物在各个阶段都会导致严重的产量损失，造成40%-50%的作物死亡。收获后储存期间真菌的变质现象经常出现，主要原因是含油量高。目前，化学消毒剂和农用化学品被广泛用作种子引发剂和/或收获后储存保护剂，以控制种子污染真菌感染。然而，合成杀菌剂的应用需要对几个参数进行实验评估，并引发了人们对杀菌剂抗性出现的严重担忧。在这方面，天然抗菌剂如微生物次生代谢产物作为一种环保且经济高效的种子保护替代品更受青睐。为此，使用2,4-二乙酰基间苯三酚等广谱抗生素可能比化学杀菌剂更有利于种子引发。在本研究中，它对产生黄曲霉毒素的黄曲霉以及其他植物病原真菌，如玉米黑粉菌、尖孢镰刀菌、核盘菌、旋孢炭疽菌和黑刺盘孢菌表现出最大抑制活性。因此，研究了其用于花生种子引发，以抑制产毒黄曲霉的增殖。在盆栽试验中，与甲醇引发和未引发的种子相比，用2,4-二乙酰基间苯三酚引发的种子在收获后能长期保存，种子发芽率和生长指标得到提高。因此，建议将2,4-二乙酰基间苯三酚引发作为一种低成本、双重效益的种子储存保护剂，用于控制油料作物收获后的真菌变质，以及作为一种植物生长刺激剂，促进长期储存后田间植物的生长。
