Liu Yang, Chen Song-Lin, Meng Liang, Zhang Yu-Xi
Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Key Lab For Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Qingdao, Shandong, China.
Fish Shellfish Immunol. 2007 Oct;23(4):711-20. doi: 10.1016/j.fsi.2007.01.008. Epub 2007 Jan 20.
Chemokines represent a superfamily of chemotactic cytokines playing an important role in leucocyte chemotaxis. Here we report a novel turbot CXC chemokine screened from a turbot spleen cDNA library. The complete cDNA of the turbot CXC chemokine contains an 81bp 5' UTR, a 414bp open reading frame (ORF) encoding 137 amino acids and a 449bp 3' UTR. Four exons and three introns are identified in the turbot CXC chemokine gene. Phylogenetic analysis showed that the turbot CXC chemokine clustered apart from all other CXC chemokines. RT-PCR demonstrated that turbot CXC chemokine was expressed highly in spleen and head kidney. During the early stages of embryo development after fertilization, it appears that low expression level of turbot CXC chemokine was firstly observed at somites stage. Interestingly, the turbot chemokine was highly and rapidly (5h) induced in liver, spleen and head kidney of turbot after challenge with Vibrio anguillarum. Furthermore, the expression of CXC chemokine was also dramatically increased after challenge in turbot embryonic cells (TECs). These results indicated that the turbot CXC chemokine played an important role in turbot immune response.
趋化因子是趋化性细胞因子的一个超家族，在白细胞趋化中起重要作用。在此，我们报道了从大菱鲆脾脏cDNA文库中筛选出的一种新型大菱鲆CXC趋化因子。大菱鲆CXC趋化因子的完整cDNA包含一个81bp的5'非翻译区、一个编码137个氨基酸的414bp开放阅读框（ORF）和一个449bp的3'非翻译区。在大菱鲆CXC趋化因子基因中鉴定出四个外显子和三个内含子。系统发育分析表明，大菱鲆CXC趋化因子与所有其他CXC趋化因子聚类不同。RT-PCR表明，大菱鲆CXC趋化因子在脾脏和头肾中高表达。在受精后的胚胎发育早期，似乎在体节期首次观察到大菱鲆CXC趋化因子的低表达水平。有趣的是，在用鳗弧菌攻击后，大菱鲆肝脏、脾脏和头肾中的大菱鲆趋化因子在5小时内迅速高表达。此外，在大菱鲆胚胎细胞（TECs）攻击后，CXC趋化因子的表达也显著增加。这些结果表明，大菱鲆CXC趋化因子在大菱鲆免疫反应中起重要作用。
