Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai 200065, China.
Fish Shellfish Immunol. 2012 Nov;33(5):1215-21. doi: 10.1016/j.fsi.2012.09.001. Epub 2012 Sep 13.
The Y-box proteins are a family of highly conserved nucleic acid binding proteins. In this report we have identified a new member, YB-1 from turbot (Scophthalmus maximus) spleen cDNA library. The full-length cDNA sequence of turbot YB-1 was obtained and then the expression at transcriptional level was researched by qRT-PCR. In normal organs, the expression of YB-1 was higher in liver, brain, gill and heart, respectively. YB-1 had the highest expression level at gastrula stage during the early stages of embryo development. In the liver, kidney and spleen, the turbot YB-1 expression level was the highest at 72 h after challenge with lymphocystis disease virus (LCDV) and the highest at 12 h after challenge with Vibrio anguillarum (V. anguillarum). Furthermore, the expression of turbot YB-1 also distinctly increased in turbot kidney cells (TK) at 24 h after challenge with V. anguillarum and LCDV. These results indicated that the turbot YB-1 protein may play a significant role in the immune response of turbot.
Y 盒蛋白是一组高度保守的核酸结合蛋白。本研究从大菱鲆脾脏 cDNA 文库中鉴定到一个新的 Y 盒蛋白成员 YB-1。获得了大菱鲆 YB-1 的全长 cDNA 序列，并通过 qRT-PCR 研究了其转录水平的表达。在正常组织中，YB-1 在肝脏、大脑、鳃和心脏中的表达水平较高。在胚胎发育的早期阶段，在原肠胚期 YB-1 的表达水平最高。在肝脏、肾脏和脾脏中，大菱鲆 YB-1 的表达水平在淋巴囊肿病病毒（LCDV）攻毒后 72 h 达到最高，在鳗弧菌（V. anguillarum）攻毒后 12 h 达到最高。此外，在鳗弧菌和 LCDV 攻毒后 24 h，大菱鲆肾脏细胞（TK）中 YB-1 的表达也明显增加。这些结果表明，大菱鲆 YB-1 蛋白可能在大菱鲆的免疫反应中发挥重要作用。
