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产气荚膜梭菌α-类毒素对异源梭菌磷脂酶C的保护作用分析

Analysis of protection afforded by a Clostridium perfringens alpha-toxoid against heterologous clostridial phospholipases C.

作者信息

Neeson Brendan N, Clark Graeme C, Atkins Helen S, Lingard Bryan, Titball Richard W

机构信息

Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK.

出版信息

Microb Pathog. 2007 Oct;43(4):161-5. doi: 10.1016/j.micpath.2007.05.004. Epub 2007 May 18.

DOI:10.1016/j.micpath.2007.05.004

PMID:17604945

Abstract

The major virulence determinant in clostridial myonecrosis caused by Clostridium perfringens is a phospholipase C (PLC), the alpha-toxin. Previously, mice have been protected against challenge with heterologous alpha-toxin or Clostridium perfringens spores by immunisation with the C-domain (known as Cpa(247-370) or alpha-toxoid) of the alpha-toxin. In this study, we have determined the ability of the alpha-toxoid to protect against the lethal effects of a divergent C. perfringens alpha-toxin and against the PLCs of C. absonum or C. bifermentans, species which have been isolated from cases of clostridial myonecrosis. Protection against the C. perfringens alpha-toxin variant, the C. absonum alpha-toxin or the C. bifermentans PLC was elicited by immunisation with the alpha-toxoid in vivo.

摘要

由产气荚膜梭菌引起的气性坏疽的主要毒力决定因素是一种磷脂酶C（PLC），即α毒素。此前，通过用α毒素的C结构域（称为Cpa(247 - 370)或α类毒素）免疫，小鼠已受到保护，可抵御异源α毒素或产气荚膜梭菌孢子的攻击。在本研究中，我们确定了α类毒素预防不同产气荚膜梭菌α毒素的致死作用以及预防从气性坏疽病例中分离出的异常梭菌或双发酵梭菌的PLC的能力。在体内用α类毒素免疫可引发针对产气荚膜梭菌α毒素变体、异常梭菌α毒素或双发酵梭菌PLC的保护作用。