Du N X
Division of Veterinary Microbiology, Nanjing Agricultural University, Jiangsu, People's Republic of China.
Rev Sci Tech. 1991 Jun;10(2):325-36.
Viral haemorrhagic disease virus (VHDV) of rabbits is a small, nonenveloped DNA virus which contains a linear, single-stranded genome approximately 7,000 bases in length. The virions are icosahedral in symmetry, consisting of 180 subunits with pentamer-hexamer clustering. The capsid is composed of four structural polypeptides: VP1:60-61 kDa, VP2:54.7 kDa, VP3:52 kDa, and VP4:26-28 kDa. VP1 is a major structural polypeptide which constitutes approximately 54.7% of the total viral proteins. There are extensive antigenic relationships among the polypeptides. Haemagglutination activity is associated with the intact virions. The genome of the VHDV isolate strain (VHDV-NJ) is partially cloned, and 4.6 kb DNA fragment of restriction mapping has been determined, showing that there are few common restriction sites compared with the restriction site mapping of the parvoviruses. There are five viral-specific mRNA species, each coding for one of the five polypeptides identified by in vitro translation. The cDNA library from viral mRNA has been established. Southern blot hybridisation shows that, to some extent, H-I virus, minute virus of mice, porcine parvovirus and goose parvovirus share homology with VHDV. However, to clarify VHDV taxonomy, further efforts must be directed towards VHDV genome analysis.
兔病毒性出血病病毒（VHDV）是一种小型无包膜DNA病毒，其基因组为线性单链，长度约7000个碱基。病毒粒子呈二十面体对称，由180个亚基组成，具有五聚体 - 六聚体聚集结构。衣壳由四种结构多肽组成：VP1：60 - 61 kDa，VP2：54.7 kDa，VP3：52 kDa，VP4：26 - 28 kDa。VP1是主要的结构多肽，约占病毒总蛋白的54.7%。这些多肽之间存在广泛的抗原关系。血凝活性与完整病毒粒子相关。VHDV分离株（VHDV - NJ）的基因组已部分克隆，并且已确定了4.6 kb的限制性图谱DNA片段，结果表明与细小病毒的限制性酶切位点图谱相比，其共同的限制性酶切位点较少。有五种病毒特异性mRNA种类，每种分别编码通过体外翻译鉴定出的五种多肽之一。已建立了病毒mRNA的cDNA文库。Southern印迹杂交表明，H - I病毒、小鼠微小病毒、猪细小病毒和鹅细小病毒在一定程度上与VHDV具有同源性。然而，为了明确VHDV的分类地位，必须进一步对VHDV基因组进行分析。
