Broly F, Marecaux P, Houdret N, Lhermitte M, Houssin R
Laboratoire de Biochimie, Hôpital Calmette, Lille, France.
Biomed Chromatogr. 1991 Nov;5(6):248-50. doi: 10.1002/bmc.1130050604.
A routine high performance liquid chromatographic method for the rapid determination of flecaïnide (Flecaine), using a novel internal standard, N-methylflecaïnide, has been developed. After deproteinization of spiked samples, flecaïnide was totally recovered at neutral pH. Flecaïnide and the internal standard were separated on a reversed phase XL 3 microns ODS column using 10 mM phosphate buffer, pH 3.0: acetonitrile (70:30) as mobile phase, in less than 10 min. With spectrofluorometric detection, the limit of quantitation for flecaïnide was 10 ng/mL. Intra- and inter-assay precision variations were 0.24% and 1.4%.
已开发出一种常规高效液相色谱法，用于快速测定氟卡尼（Flecaine），该方法使用新型内标N - 甲基氟卡尼。加标样品经去蛋白处理后，在中性pH条件下氟卡尼可完全回收。氟卡尼和内标在反相XL 3微米ODS柱上分离，以10 mM pH 3.0的磷酸盐缓冲液：乙腈（70:30）作为流动相，分离时间不到10分钟。采用荧光分光光度检测法，氟卡尼的定量限为10 ng/mL。批内和批间精密度变异分别为0.24%和1.4%。
