Marko V, Bauerová K
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Biomed Chromatogr. 1991 Nov;5(6):256-61. doi: 10.1002/bmc.1130050606.
A gas-liquid chromatographic method for the determination of pentoxifylline and its secondary alcohol metabolite in serum has been developed. The method is based on the combination of solid phase extraction, capillary column separation and nitrogen-phosphorus detection of the analytes. Optimization of the solid phase extraction conditions permitted a low concentration determination, with limits of determination of 2 ng/mL and 10 ng/mL for pentoxifylline and its metabolite, respectively. The simplicity and rapidity of the extraction step was preserved.
已开发出一种气相色谱法，用于测定血清中的己酮可可碱及其仲醇代谢物。该方法基于固相萃取、毛细管柱分离以及对分析物的氮磷检测相结合。固相萃取条件的优化使得能够进行低浓度测定，己酮可可碱及其代谢物的测定限分别为2 ng/mL和10 ng/mL。萃取步骤的简便性和快速性得以保留。
