Ostrovská V, Pechová A, Kovácová D, Svobodová X, Kusala S
Výskumný ústav lieciv, Modra.
Cesk Farm. 1990 Jun;39(4):158-60.
The present paper describes a rapid, reliable and simple method of pentoxiphyllin in plasma. The worked-out one-step extraction with dichloromethane yields 63% recovery of pentoxiphyllin, 58% recovery of the metabolite and 54% recovery of the internal standard. The employed column with a reverse load of Spherisorb ODS, mobile phase 65% of 0.01 M KH2PO4 and 35% acetonitrile, detection at the wavelength lambda = 273 nm, makes it possible to achieve determination of pentoxiphyllin at the levels commencing with 5 ng.ml-1 of plasma. Reproducibility of determination is 6.8%, or 4.5% in the pentoxiphyllin concentrations of 50, or 100 ng.ml-1.
本文描述了一种快速、可靠且简便的血浆中己酮可可碱检测方法。采用二氯甲烷一步萃取法，己酮可可碱的回收率为63%，其代谢物的回收率为58%，内标物的回收率为54%。使用装有反相负载的Spherisorb ODS柱，流动相为65%的0.01 M KH2PO4和35%的乙腈，在波长λ = 273 nm处进行检测，可实现血浆中起始浓度为5 ng.ml-1的己酮可可碱的测定。测定的重现性为6.8%，在己酮可可碱浓度为50或100 ng.ml-1时重现性为4.5%。
