Nuñez Yarelis Ortíz, Salabarria Iraida Spengler, Collado Isidro G, Hernández-Galán Rosario
Instituto de Investigaciones Fundamentales en Agricultura Tropical Alejandro de Humboldt (INIFAT), Calle:2, Esq.1, Santiago de las Vegas, CP 17200, Cuba.
Phytochemistry. 2007 Oct;68(19):2409-14. doi: 10.1016/j.phytochem.2007.05.030. Epub 2007 Jul 2.
Bioassay-guided fractionation of ethanolic extract from the wood of Juniperus lucayana afforded three sesquiterpenes named 3-hydroxypseudowiddran-6(7)-en-4-ol (1), 15-hydroxyallo-cedrol (2) and 12-hydroxywiddrol (3) together with six known sesquiterpenes (4-9) and two known flavonoids (10 and 11). Their structures were established on the basis of comprehensive spectroscopic analyses, including 2D NMR spectroscopy and mass spectrometry. The structures of compounds were identified as 1alpha,4beta,11alpha,11beta-tetramethylbicyclo[5,4,0]undec-6(7)-en-3alpha, 4alpha-diol (1), 4beta-hydroxymethyl-5,5,9beta-trimethyltricyclo[4.3.0.2(1.4)]undecan-3alpha-ol (2) and 4beta-hydroxymethyl-7alpha,11alpha,11beta-trimethylbicyclo [5.4.0]undec-1-en-4alpha-ol (3). The major compounds isolated were evaluated for their antifungal activity against Botrytis cinerea. Widdrol (7) was the most active, reaching the 71% inhibition level on mycelial growth after 6 days.
对墨西哥刺柏木材乙醇提取物进行生物测定导向的分级分离，得到了三种倍半萜，分别为3-羟基假维德兰-6(7)-烯-4-醇（1）、15-羟基别雪松醇（2）和12-羟基维德醇（3），以及六种已知的倍半萜（4 - 9）和两种已知的黄酮类化合物（10和11）。它们的结构是基于包括二维核磁共振光谱和质谱在内的综合光谱分析确定的。化合物的结构被鉴定为1α,4β,11α,11β-四甲基双环[5,4,0]十一碳-6(7)-烯-3α,4α-二醇（1）、4β-羟甲基-5,5,9β-三甲基三环[4.3.0.2(1.4)]十一烷-3α-醇（2）和4β-羟甲基-7α,11α,11β-三甲基双环[5.4.0]十一碳-1-烯-4α-醇（3）。对分离得到的主要化合物进行了抗灰葡萄孢菌的抗真菌活性评估。维德醇（7）活性最高，6天后对菌丝生长的抑制水平达到71%。
