Wang Hongjie, Lin Guiting, Zhang Zhiwen
Department of Physiology and Pathophysiology, Peking University School of Basic Medical Science, Beijing 100083, China.
Cell Biol Int. 2007 Nov;31(11):1309-15. doi: 10.1016/j.cellbi.2007.05.002. Epub 2007 May 21.
The activating transcription factor (ATF) family comprises a group of basic region-leucine zipper (bZIP) proteins, which have roles in development, differentiation, proliferation and apoptosis. Here we described that ATF5 could promote cell survival in heat shock stress and identified that heat shock protein 27 (Hsp27) as one of its target gene related to cell survival. The ectopic expression of ATF5 could significantly increase cell tolerance to elevated temperature and the CRE motif played an important role in upregulating Hsp27 gene promoter activity by ATF5. Also, inhibition of Hsp27 expression by RNAi could increase cell death in ATF5 ectopic expressing cells, indicating that Hsp27 involved in ATF5 mediated cell survival. Taken together, our results indicated that ATF5 promoted H9c2 cell survival in heat shock stress at least through activation of Hsp27.
激活转录因子（ATF）家族由一组碱性区域-亮氨酸拉链（bZIP）蛋白组成，它们在发育、分化、增殖和凋亡中发挥作用。在此我们描述了ATF5可在热休克应激中促进细胞存活，并确定热休克蛋白27（Hsp27）是其与细胞存活相关的靶基因之一。ATF5的异位表达可显著提高细胞对高温的耐受性，且CRE基序在ATF5上调Hsp27基因启动子活性中起重要作用。此外，通过RNAi抑制Hsp27表达可增加ATF5异位表达细胞的死亡，表明Hsp27参与了ATF5介导的细胞存活。综上所述，我们的结果表明ATF5至少通过激活Hsp27促进H9c2细胞在热休克应激中的存活。
