Gore A C, Terasawa E
Wisconsin Regional Primate Research Center, Madison 53715.
Brain Res. 1991 Sep 27;560(1-2):268-75. doi: 10.1016/0006-8993(91)91242-s.
In order to examine the neurophysiological properties of the hypothalamus responsible for the pulsatile release of luteinizing hormone (LH) the medial basal hypothalamus (MBH) of the ovariectomized adult female guinea pig was stimulated electrically through an implanted electrode and LH release was monitored as an indication of luteinizing hormone-releasing hormone (LHRH) release. Electrical stimulation (ES) consisting of monophasic square wave pulses with 0.5 ms pulse duration was applied for 2 min at 60, 40, 20, or 10 min intervals. Current intensity (80, 200, 500 microA) and frequency (8, 50, 100 Hz) were varied to determine the parameters of ES which resulted in an LH pulse with amplitude similar to that of a pulse induced by the endogenous pulse-generating mechanism. Blood samples were collected at 5 or 10 min intervals through an indwelling catheter, and LH was measured by RIA.(ABSTRACT TRUNCATED AT 250 WORDS)
为了研究负责促黄体生成素（LH）脉冲式释放的下丘脑神经生理特性，通过植入电极对成年去卵巢雌性豚鼠的内侧基底下丘脑（MBH）进行电刺激，并监测LH释放以作为促性腺激素释放激素（LHRH）释放的指标。电刺激（ES）由脉冲持续时间为0.5毫秒的单相方波脉冲组成，以60、40、20或10分钟的间隔施加2分钟。改变电流强度（80、200、500微安）和频率（8、50、100赫兹），以确定能产生与内源性脉冲发生机制诱导的脉冲幅度相似的LH脉冲的电刺激参数。通过留置导管每隔5或10分钟采集血样，并用放射免疫分析法测定LH。（摘要截取自250字）
