Molina J R, Hard D L, Anderson L L
Biol Reprod. 1986 Sep;35(2):439-46. doi: 10.1095/biolreprod35.2.439.
The control of luteinizing hormone (LH) secretion was investigated in ovariectomized, prepubertal Yorkshire pigs by comparing the effects of anterior (AHD), complete (CHD), and posterior (PHD) hypothalamic deafferentation to sham-operated controls (SOC). Gilts (n = 16) were assigned randomly to treatments, fitted with an indwelling jugular catheter, and ovariectomized 2 days before deafferentation or sham-operation (Day 0). Blood for radioimmunoassay (RIA) of LH was collected sequentially at 20-min intervals for a period of 2 h before and 24, 48, 72, and 96 h after hypothalamic deafferentation or SOC. Episodic LH release after AHD or CHD was abolished (p less than 0.01), but not after PHD or SOC. Concentrations of serum LH in AHD and CHD dropped (p less than 0.01) at 24 and 48 h after surgery. Levels of LH before and after surgery in PHD and SOC were similar (p greater than 0.05). Infusion of 25 micrograms LH-releasing hormone (LHRH) i.v. at 72 and 96 h after hypothalamic deafferentation and SOC increased (p less than 0.01) serum LH to peak levels within 15 min. after infusion; LH returned to basal levels 60-80 min later. By 96 h after surgery, LH response to LH-releasing hormone (LHRH) was less in AHD and CHD as compared with the response at 72 h postinjection. Concentrations of LH in PHD and SOC were similar (p greater than 0.05) at 72 and 96 h, respectively. The results from this study clearly indicate that neural stimuli originating or traversing the neural areas rostral to the median eminence are required for secretion of LH in the pig.(ABSTRACT TRUNCATED AT 250 WORDS)
通过比较下丘脑前侧去传入（AHD）、完全去传入（CHD）和后侧去传入（PHD）对假手术对照（SOC）的影响，研究了去卵巢的青春期前约克夏猪促黄体生成素（LH）分泌的控制情况。将16头后备母猪随机分配至各处理组，安装颈内留置导管，并在去传入或假手术（第0天）前2天进行卵巢切除。在下丘脑去传入或SOC之前2小时以及之后24、48、72和96小时，每隔20分钟依次采集用于LH放射免疫测定（RIA）的血液。AHD或CHD后LH的阵发性释放被消除（p<0.01），但PHD或SOC后未被消除。AHD和CHD组血清LH浓度在手术后24和48小时下降（p<0.01）。PHD和SOC组手术前后LH水平相似（p>0.05）。在下丘脑去传入和SOC后72和96小时静脉注射25微克促黄体生成素释放激素（LHRH），可使血清LH在注射后15分钟内升高至峰值水平（p<0.01）；LH在60 - 80分钟后恢复至基础水平。到手术后96小时，与注射后72小时相比，AHD和CHD组LH对促黄体生成素释放激素（LHRH）的反应降低。PHD和SOC组在72和96小时时LH浓度分别相似（p>0.05）。本研究结果清楚地表明，猪LH分泌需要起源于或穿过正中隆起前方神经区域的神经刺激。（摘要截断于250字）
