Reid Jennifer D, Parker Carol E, Borchers Christoph H
University of Victoria Genome BC Proteomics Centre, Vancouver Island Technology Park, 3101-4464 Markham Street, Victoria, BC V8Z 7X8, Canada.
Curr Opin Mol Ther. 2007 Jun;9(3):216-21.
The value of biomarker research in the field of diagnostics and predictive medicine has long been acknowledged. Diagnostic tests have evolved from single-molecule monitoring to multiplexed assays for entire panels of biomarkers, resulting in comprehensive data that clinicians can use to efficiently and effectively treat patients. Thus, the focus of protein microarrays has been directed toward the development of high-throughput multiplex assays that are capable of producing highly accurate quantitative data for a dynamic range of molecules to meet the requirements of the clinical laboratory. Some recent advancements in the field of biomarker discovery, including mass-spectrometry based approaches, as well as the advantages and limitations of the various techniques, will be discussed in this review.
生物标志物研究在诊断和预测医学领域的价值早已得到认可。诊断测试已从单分子监测发展到针对整个生物标志物面板的多重检测，从而产生临床医生可用于高效、有效治疗患者的全面数据。因此，蛋白质微阵列的重点已转向开发高通量多重检测方法，这些方法能够为一系列动态分子生成高度准确的定量数据，以满足临床实验室的要求。本文将讨论生物标志物发现领域的一些最新进展，包括基于质谱的方法，以及各种技术的优缺点。
