Lin Shan, Zheng Wei-wen, Wu Jin-zhong, Zhou Li-juan, Song Ya-na
Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China.
Zhongguo Zhong Yao Za Zhi. 2007 Apr;32(8):671-5.
To provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats.
To compare nrDNA-ITS base sequence using specific PCR-ITS.
The completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found.
The method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences.
通过探究不同产地莲的nrDNA-ITS序列差异，为莲的鉴定提供DNA分子标记。
采用特异性PCR-ITS比较nrDNA-ITS碱基序列。
获得了莲的ITS和5.8 S rDNA完整序列以及18S rDNA和26S rDNA部分序列，共750 bp。发现了不同产地和不同品种莲之间的差异。
该方法可用于不同种莲的鉴别及伪品区分，为通过比较ITS序列鉴定不同地理区域的莲提供了依据。
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