Liu Wen-Zhi, Dai Zhu-Bo, Qian Zi-Gang
Yunan College of Traditional Chinese Medicine, Kunming 650200, China.
Zhong Yao Cai. 2008 Feb;31(2):192-5.
To analyze the ribosomal ITS sequence variation of Psammosilene tuncolides W. C. Wu et C. Y. Wu from different populations, for identifying different local populations.
A pair of primers of 18SP1 and 26SP2 with PCR technique had been applied to study the ITS sequences.
The sequences of ITS1, ITS2 and 5.8S are 225-229 bp, 166-170 bp and 261-264 bp. Among 12 local populations, the sequence of Kunming, Lijiang, Gejiu, Heqing of Yunnan and Yanyuan of Sichuan showed no variation, there were 1-4 variable sites (including 5.8S coding region) in pairwise comparison of the other 7 local populations, including Xuanwei, Huize, Zhongdian, Baoshan of Yunnan, Muli of Sichuan and Linzhi of Tibet.
Comparative analysis shows that the ITS sequences of different local populations in the middle of Yunnan, southwest of Sichuan and west, northwest of Yunnan, southeast of Tibet, southwest of Sichuan have different fingerprint character, so the ITS sequences can be used to identify different local populations. The variation of ITS sequence of Psammosilene tunicoides is related to its geographical distribution.
分析不同居群金铁锁核糖体ITS序列变异情况，以鉴别不同居群。
采用18SP1和26SP2引物对，运用PCR技术研究ITS序列。
ITS1、ITS2及5.8S序列长度分别为225 - 229bp、166 - 170bp和261 - 264bp。12个居群中，云南昆明、丽江、个旧、鹤庆及四川盐源居群序列无变异，云南宣威、会泽、中甸、保山，四川木里及西藏林芝等7个居群两两比较有1 - 4个变异位点（含5.8S编码区）。
比较分析表明，滇中、滇西南、滇西北、藏东南、川西南等地不同居群金铁锁ITS序列具有不同指纹特征，可用于鉴别不同居群。金铁锁ITS序列变异与其地理分布有关。
