Parkinson John S
Department of Biology, University of Utah, Salt Lake City, UT, USA.
Methods Enzymol. 2007;423:432-5. doi: 10.1016/S0076-6879(07)23020-4.
The morphologies of bacterial colonies in soft agar media can provide a wealth of information about a strain's locomotor and chemotactic abilities. Photographic images are often the simplest and most effective means of documenting these behavioral phenotypes. Uniform, indirect, transmitted illumination of the plates is essential for obtaining good colony images. This brief chapter describes a simple and relatively inexpensive illumination device for viewing and photographing bacterial colonies in soft agar.
软琼脂培养基中细菌菌落的形态可以提供有关菌株运动和趋化能力的丰富信息。照片图像通常是记录这些行为表型的最简单、最有效的方法。平板的均匀、间接透射照明对于获得良好的菌落图像至关重要。本章简要介绍一种简单且相对便宜的照明装置,用于观察和拍摄软琼脂中的细菌菌落。