倾注琼脂平板并划线或涂布以分离单个菌落。
Pouring agar plates and streaking or spreading to isolate individual colonies.
作者信息
Eyler Elizabeth
机构信息
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
出版信息
Methods Enzymol. 2013;533:3-14. doi: 10.1016/B978-0-12-420067-8.00001-5.
Abstract
Agar plates, composed of growth media solidified with agar, are commonly used to culture yeast and bacteria. Cultures may be spread or streaked across the surface of these plates to facilitate titer calculations or to permit isolation of single, genetically identical colonies.
摘要
由琼脂固化的生长培养基组成的琼脂平板通常用于培养酵母和细菌。培养物可以铺展或划线接种在这些平板的表面，以方便进行滴度计算或分离单个基因相同的菌落。
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