Joseph Biju, Beier Dagmar
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Würzburg, Germany.
Methods Enzymol. 2007;423:514-30. doi: 10.1016/S0076-6879(07)23025-3.
The human gastric pathogen Helicobacter pylori was among the first microorganisms whose genome sequence was determined. It has a remarkably small repertoire of two-component regulators comprising three histidine kinases and five response regulators involved in transcriptional regulators as well as a bifunctional histidine kinase and four response regulators which build up the chemotaxis regulatory system. However, the two-component systems of H. pylori proved to play an important role for both in vitro growth of the organism and its ability to colonize its host. Here, we describe the experimental approaches applied to characterize the two-component systems of H. pylori, which were mostly based on the availability of the H. pylori genome sequence. These approaches comprise conventional techniques including mutation analysis as well as sophisticated methods like whole genome transcriptional profiling.
人类胃部病原体幽门螺杆菌是最早一批测定其基因组序列的微生物之一。它拥有极为有限的双组分调节系统，包括三个组氨酸激酶和五个参与转录调节的响应调节因子，以及一个构建趋化调节系统的双功能组氨酸激酶和四个响应调节因子。然而，幽门螺杆菌的双组分系统已被证明对该生物体的体外生长及其定殖于宿主的能力均起着重要作用。在此，我们描述了用于表征幽门螺杆菌双组分系统的实验方法，这些方法大多基于幽门螺杆菌基因组序列的可得性。这些方法包括传统技术，如突变分析，以及复杂的方法，如全基因组转录谱分析。
