Thachil Anil J, Velayudhan Binu T, Lopes-Berkas Vanessa C, Halvorson David A, Nagaraja Kakambi V
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
J Vet Diagn Invest. 2007 Jul;19(4):417-20. doi: 10.1177/104063870701900415.
Ornithobacterium rhinotracheale (ORT) is an infectious respiratory pathogen of chickens, turkeys, and wild birds. There are 18 serotypes of ORT reported worldwide. In this study, enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction and random amplified polymorphic DNA assay with Universal M13 primer-based fingerprinting techniques were investigated for their ability to differentiate ORT isolates. The authors examined 50 field isolates and 8 reference strains of ORT for their genetic differences. The fingerprint patterns were compared with serotyping results of ORT by the agar gel precipitation test. M13 fingerprinting revealed different patterns for 6 reference serotypes of ORT that were tested, namely, C, D, E, I, J, and K. Ornithobacterium rhinotracheale reference serotypes A and F yielded indistinguishable fingerprints with M13 fingerprinting. The ERIC 1R technique discerned only 5 of the 8 reference serotypes of ORT. Distinct fingerprints were also found within the ORT serotypes with both techniques. From 58 isolates of ORT that were fingerprinted belonging to 8 ORT serotypes, 10 different fingerprints were obtained with M13 fingerprinting and 6 different fingerprints were obtained with ERIC 1R fingerprinting. M13 fingerprinting technique was found to be more discriminative in differentiating ORT isolates than the ERIC 1R fingerprinting technique. These results suggest that fingerprinting techniques may be a more discerning tool for characterizing ORT isolates than the serological test using the agar gel precipitation test. This fingerprinting technique could potentially be a valuable tool in identifying an isolate from a clinical outbreak of ORT infection for development of an autogenous vaccine.
禽气管鸟杆菌（ORT）是鸡、火鸡和野生鸟类的一种传染性呼吸道病原体。全球已报道有18种ORT血清型。在本研究中，对肠杆菌重复基因间共有序列（ERIC）聚合酶链反应以及基于通用M13引物指纹技术的随机扩增多态性DNA分析区分ORT分离株的能力进行了研究。作者检测了50株ORT野外分离株和8株参考菌株的遗传差异。通过琼脂凝胶沉淀试验将指纹图谱与ORT的血清分型结果进行比较。M13指纹分析显示，所检测的ORT的6种参考血清型（即C、D、E、I、J和K）呈现出不同的图谱。ORT参考血清型A和F通过M13指纹分析产生无法区分的指纹图谱。ERIC 1R技术仅能区分8种ORT参考血清型中的5种。两种技术在ORT血清型内也发现了不同的指纹图谱。在所进行指纹分析的属于8种ORT血清型的58株ORT分离株中，M13指纹分析获得了10种不同的指纹图谱，ERIC 1R指纹分析获得了6种不同的指纹图谱。结果发现M13指纹分析技术在区分ORT分离株方面比ERIC 1R指纹分析技术更具鉴别力。这些结果表明，与使用琼脂凝胶沉淀试验的血清学检测相比，指纹分析技术可能是一种更具鉴别力的ORT分离株鉴定工具。这种指纹分析技术有可能成为从ORT感染临床暴发中鉴定分离株以开发自家疫苗的一种有价值的工具。
