一只猫的轻链多发性骨髓瘤。

Light-chain multiple myeloma in a cat.

作者信息

Yamada Osamu, Tamura Kyoichi, Yagihara Hiroko, Isotani Mayu, Azakami Mari, Sawada Satoko, Ono Kenichiro, Washizu Tsukimi, Bonkobara Makoto

机构信息

Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.

出版信息

J Vet Diagn Invest. 2007 Jul;19(4):443-7. doi: 10.1177/104063870701900421.

DOI:10.1177/104063870701900421

PMID:17609361

Abstract

A diagnosis of light-chain multiple myeloma was made in an 11-year-old male American Shorthair cat. The cat showed atypical plasma cell infiltration in the bone marrow, biclonal gammopathy caused by polymerization of myeloma protein (M-protein), and Bence-Jones proteinuria. The M-protein in the serum of the cat was analyzed by using 12% sodium dodeyl sulfate (SDS) polyacrylamide gel electrophoresis with Coomassie brilliant blue staining. An intense band with a size of 27 kDa, the size of the immunoglobulin light chain, was clearly observed, whereas the band corresponding to the immunoglobulin heavy chain (59 kDa) was undetectable. The 27-kDa band was confirmed to be an immunoglobulin light chain by Western blotting by using antibodies for feline immunoglobulin. These data suggested that the neoplastic plasma cells produce light chain only, leading to the diagnosis of light-chain multiple myeloma in the cat.

摘要

一只11岁的美国短毛雄性猫被诊断为轻链多发性骨髓瘤。该猫骨髓中出现非典型浆细胞浸润，骨髓瘤蛋白（M蛋白）聚合导致双克隆丙种球蛋白病，以及本周蛋白尿。采用12%十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳和考马斯亮蓝染色法分析该猫血清中的M蛋白。清晰观察到一条大小为27 kDa（免疫球蛋白轻链大小）的强条带，而对应免疫球蛋白重链（59 kDa）的条带未检测到。通过使用针对猫免疫球蛋白的抗体进行蛋白质印迹法，证实27 kDa条带为免疫球蛋白轻链。这些数据表明肿瘤性浆细胞仅产生轻链，从而诊断该猫为轻链多发性骨髓瘤。