利用拜氏梭菌从麦秸水解物中生产丁醇。
Butanol production from wheat straw hydrolysate using Clostridium beijerinckii.
作者信息
Qureshi Nasib, Saha Badal C, Cotta Michael A
机构信息
United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Fermentation Biotechnology Research Unit, 1815 N University Street, Peoria, IL 61604, USA.
出版信息
Bioprocess Biosyst Eng. 2007 Nov;30(6):419-27. doi: 10.1007/s00449-007-0137-9. Epub 2007 Jul 3.
In these studies, butanol (acetone butanol ethanol or ABE) was produced from wheat straw hydrolysate (WSH) in batch cultures using Clostridium beijerinckii P260. In control fermentation 48.9 g L(-1) glucose (initial sugar 62.0 g L(-1)) was used to produce 20.1 g L(-1) ABE with a productivity and yield of 0.28 g L(-1 )h(-1) and 0.41, respectively. In a similar experiment where WSH (60.2 g L(-1) total sugars obtained from hydrolysis of 86 g L(-1) wheat straw) was used, the culture produced 25.0 g L(-1) ABE with a productivity and yield of 0.60 g L(-1 )h(-1) and 0.42, respectively. These results are superior to the control experiment and productivity was improved by 214%. When WSH was supplemented with 35 g L(-1) glucose, a reactor productivity was improved to 0.63 g L(-1 )h(-1) with a yield of 0.42. In this case, ABE concentration in the broth was 28.2 g L(-1). When WSH was supplemented with 60 g L(-1) glucose, the resultant medium containing 128.3 g L(-1) sugars was successfully fermented (due to product removal) to produce 47.6 g L(-1) ABE, and the culture utilized all the sugars (glucose, xylose, arabinose, galactose, and mannose). These results demonstrate that C. beijerinckii P260 has excellent capacity to convert biomass derived sugars to solvents and can produce over 28 g L(-1) (in one case 41.7 g L(-1) from glucose) ABE from WSH. Medium containing 250 g L(-1) glucose resulted in no growth and no ABE production. Mixtures containing WSH + 140 g L(-1) glucose (total sugar approximately 200 g L(-1)) showed poor growth and poor ABE production.
在这些研究中，使用拜氏梭菌P260在分批培养中从麦秸水解物（WSH）生产丁醇（丙酮丁醇乙醇或ABE）。在对照发酵中，使用48.9 g L⁻¹葡萄糖（初始糖62.0 g L⁻¹）生产20.1 g L⁻¹ ABE，生产率和产率分别为0.28 g L⁻¹ h⁻¹和0.41。在类似的实验中，使用WSH（从86 g L⁻¹麦秸水解得到的总糖60.2 g L⁻¹），培养物生产了25.0 g L⁻¹ ABE，生产率和产率分别为0.60 g L⁻¹ h⁻¹和0.42。这些结果优于对照实验，生产率提高了214%。当向WSH中添加35 g L⁻¹葡萄糖时，反应器生产率提高到0.63 g L⁻¹ h⁻¹，产率为0.42。在这种情况下，肉汤中的ABE浓度为28.2 g L⁻¹。当向WSH中添加60 g L⁻¹葡萄糖时，所得含有128.3 g L⁻¹糖的培养基成功发酵（由于产物去除）以生产47.6 g L⁻¹ ABE，并且培养物利用了所有的糖（葡萄糖、木糖、阿拉伯糖、半乳糖和甘露糖）。这些结果表明，拜氏梭菌P260具有将生物质衍生的糖转化为溶剂的优异能力，并且可以从WSH生产超过28 g L⁻¹（在一种情况下从葡萄糖生产41.7 g L⁻¹）的ABE。含有250 g L⁻¹葡萄糖的培养基没有生长且没有ABE产生。含有WSH + 140 g L⁻¹葡萄糖（总糖约200 g L⁻¹）的混合物生长不良且ABE产量低。
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