Przybysz Magdalena, Maszczak Dorota, Borysewicz Krzysztof, Szechiński Jacek, Katnik-Prastowska Iwona
Department of Chemistry and Immunochemistry, Wrocław Medical University, Bujwida 44a, 50-345, Wrocław, Poland.
Glycoconj J. 2007 Dec;24(9):543-50. doi: 10.1007/s10719-007-9049-9. Epub 2007 Jul 3.
The expressions of terminal sugars in synovial and plasma fibronectins were studied in relation to rheumatoid arthritis (RA) progression defined according to the early, established and late radiological changes in the patients' hands. The relative amounts of sialic acid and fucose were analyzed by lectin-ELISA using appropriate sialic acid-linked alpha2-3 (Maackia amurensis) and alpha2-6 (Sambucus nigra) lectins as well as fucose-linked alpha1-6 (Aleuria aurantia), alpha1-2 (Ulex europaeus), and alpha1-3 (Tetragonolobus purpureus). In the early RA group, the synovial fibronectin reactivities were the lowest with the all lectins used. In the established and late groups, relative sialylation and fucosylation significantly increased. However, sialylation negligibly decreased, whereas fucosylation remained at nearly the same level in the late group. Moreover, the expression of alpha1-6-linked fucose was found to be related to disease activity. In contrast, plasma fibronectin reactivity with lectins showed different dynamic alterations. In the early RA group, the reactivity of fibronectin with the lectins used was similar to that of healthy individuals, whereas it increased significantly in the established RA group compared with the early and normal plasma groups. In the late RA group it decreased to a level similar to that of the normal group. The lower expressions of terminal sugars in synovial fibronectin were mainly associated with the early degenerative processes of RA. In conclusion, such alterations may be applicable as a stage-specific marker for diagnosis and therapy of RA patients. The higher expression of terminal sugars in fibronectin could be associated with repair and adaptation processes in longstanding disease.
研究了滑膜和血浆纤连蛋白中末端糖的表达与类风湿关节炎（RA）进展的关系，RA进展根据患者手部早期、已确诊和晚期的放射学变化来定义。使用适当的与唾液酸相连的α2-3（黑果越桔）和α2-6（接骨木）凝集素以及与岩藻糖相连的α1-6（橙黄网孢盘菌）、α1-2（荆豆）和α1-3（紫花四角豆）凝集素，通过凝集素酶联免疫吸附测定法分析唾液酸和岩藻糖的相对含量。在早期RA组中，滑膜纤连蛋白与所有使用的凝集素的反应性最低。在已确诊和晚期组中，相对唾液酸化和岩藻糖化显著增加。然而，在晚期组中，唾液酸化略有下降，而岩藻糖化水平几乎保持不变。此外，发现α1-6连接的岩藻糖的表达与疾病活动有关。相比之下，血浆纤连蛋白与凝集素的反应性表现出不同的动态变化。在早期RA组中，纤连蛋白与所使用凝集素的反应性与健康个体相似，而在已确诊的RA组中，与早期和正常血浆组相比，其反应性显著增加。在晚期RA组中，其降至与正常组相似的水平。滑膜纤连蛋白中末端糖的较低表达主要与RA的早期退行性过程有关。总之，这种改变可能作为RA患者诊断和治疗的阶段特异性标志物。纤连蛋白中末端糖的较高表达可能与长期疾病中的修复和适应过程有关。
