Department of Chemistry and Immunochemistry, Wrocław Medical University, Poland.
Dis Markers. 2011;31(5):317-25. doi: 10.3233/DMA-2011-0846.
The aim of this study was to compare fucose and sialic acid residue expression on fibronectin and α{1}-acid glycoprotein in the seminal plasma of men suspected of infertility and suffering from leukocytospermia.
Seminal ejaculates were collected from 27 leukocytospermic and 18 healthy, normozoospermic men. The relative degree of fucosylation and sialylation of fibronectin and α{1}-acid glycoprotein was estimated by ELISA using fucose and sialic acid specific lectins from Aleuria aurantia, Lotus tetragonolobus, and Ulex europaeus as well as Maackia amurensis and Sambucus nigra, respectively.
Leukocytospermic seminal fibronectin, in comparison with fibronectin of normal fertile group, showed lower relative reactivity with AAL, LTA and UEA, and higher reactivity with MAA and SNA, while the AGP of the leukocytospermic group was less reactive with AAL, and the relative reactivity with LTA and MAA was significantly higher. Fibronectin and α{1}-acid glycoprotein reactivity with UEA and MAA showed high positive correlations.
Leukocytospermia was associated with the alterations of terminal monosaccharide expression in human seminal fibronectin and α{1}-acid glycoprotein. The increase of sialyl-Lewis{ x} antigen in α{1}-acid glycoprotein can be used as a marker of genital tract inflammation manifested by leukocytospermia.
本研究旨在比较怀疑不育且患有白细胞精子症的男性的精液纤维连接蛋白和α{1}-酸性糖蛋白中岩藻糖和唾液酸残基的表达。
收集了 27 名白细胞精子症患者和 18 名健康、正常精子症患者的精液样本。通过使用来自 Aleuria aurantia、Lotus tetragonolobus 和 Ulex europaeus 的岩藻糖和唾液酸特异性凝集素以及 Maackia amurensis 和 Sambucus nigra 来估计纤维连接蛋白和α{1}-酸性糖蛋白的岩藻糖基化和唾液酸化的相对程度。
白细胞精子症患者的精液纤维连接蛋白与正常生育组的纤维连接蛋白相比，与 AAL、LTA 和 UEA 的相对反应性较低，与 MAA 和 SNA 的反应性较高，而白细胞精子症组的 AGP 与 AAL 的反应性较低，与 LTA 和 MAA 的相对反应性显著升高。纤维连接蛋白和α{1}-酸性糖蛋白与 UEA 和 MAA 的反应性呈高度正相关。
白细胞精子症与人类精液纤维连接蛋白和α{1}-酸性糖蛋白末端单糖表达的改变有关。α{1}-酸性糖蛋白中唾液酸化-Lewis{ x}抗原的增加可作为白细胞精子症表现的生殖道炎症的标志物。
