Callera Fernando, de Melo Cláudio M T P
Serviço de Hematologia e Hemoterapia de São José Dos Campos, São José Dos Campos-São Paulo, CEP 12210-040, Brasil.
Stem Cells Dev. 2007 Jun;16(3):461-6. doi: 10.1089/scd.2007.0083.
The purpose of this study was to demonstrate the possibility of delivering autologous bone marrow precursor cells into the spinal cord via lumbar puncture technique (LP) in patients with spinal cord injury (SCI). Magnetic resonance imaging provides a noninvasive method for studying the fate of transplanted cells in vivo. Considering these propositions, we studied magnetic resonance tracking of autologous bone marrow CD34(+) cells labeled with magnetic nanoparticles delivered into the spinal cord via LP in patients with SCI. Sixteen patients with chronic SCI were enrolled and divided into two groups; one group got their own labeled-CD34(+) cells injected into the spinal cord via LP (n = 10); the others received an injection, but it contained magnetic beads without stem cells (controls, n = 6). CD34(+) cells were magnetically labeled with magnetic beads coated with a monoclonal antibody specific for the CD34 cell membrane antigen. Magnetic resonance images were obtained by a standard turbospin echo-T2 weighted sequences before and 20 and 35 days after post-transplantation. The median number of CD34(+) cells injected via LP was 0.7 x 10(6) (range 0.45 to 1.22 x 10(6)). Magnetically labeled CD34(+) cells were visible at the lesion site as hypointense signals in five patients of the labeled-CD34(+) group 20 and 35 days after transplantation; these signals were not visible in any patient of the control group. We suggested for the first time that autologous bone marrow CD34(+) cells labeled with magnetic nanoparticles delivered into the spinal cord via LP technique migrated into the injured site in patients with chronic SCI.
本研究的目的是证明在脊髓损伤（SCI）患者中，通过腰椎穿刺技术（LP）将自体骨髓前体细胞输送到脊髓的可能性。磁共振成像提供了一种在体内研究移植细胞命运的非侵入性方法。考虑到这些因素，我们研究了在SCI患者中，通过LP将用磁性纳米颗粒标记的自体骨髓CD34(+)细胞输送到脊髓后的磁共振追踪情况。16例慢性SCI患者被纳入研究并分为两组；一组通过LP将他们自己标记的CD34(+)细胞注入脊髓（n = 10）；另一组接受注射，但注射物含有不含干细胞的磁珠（对照组，n = 6）。CD34(+)细胞用包被有针对CD34细胞膜抗原的单克隆抗体的磁珠进行磁性标记。在移植前以及移植后20天和35天，通过标准的快速自旋回波-T2加权序列获得磁共振图像。通过LP注入的CD34(+)细胞的中位数为0.7×10(6)（范围为0.45至1.22×10(6)）。在移植后20天和35天，在标记-CD34(+)组的5例患者中，磁性标记的CD34(+)细胞在损伤部位可见为低信号；在对照组的任何患者中均未见到这些信号。我们首次表明，通过LP技术将用磁性纳米颗粒标记的自体骨髓CD34(+)细胞输送到脊髓后，这些细胞在慢性SCI患者中迁移到了损伤部位。
