Ward R J, Florence A L, Baldwin D, Abiaka C, Roland F, Ramsey M H, Dickson D P, Peters T J, Crichton R R
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, England.
Eur J Biochem. 1991 Dec 5;202(2):405-10. doi: 10.1111/j.1432-1033.1991.tb16389.x.
Male Wistar rats fed with ferrocene had high hepatic iron loading (7.24 +/- 1.97 mg Fe/g tissue) after 6 weeks, principally located in lysosomes, which was comparable to the levels and distribution determined in human haemochromatosis. The two iron-storage proteins, ferritin and haemosiderin were isolated from the livers of the ferrocene-loaded rats and their iron cores were investigated by Mössbauer spectroscopy and inductively coupled plasma-emission spectrometry. Ferrihydrite was the predominant form of iron present in both ferritin and haemosiderin, while haemosiderin contained higher amounts of phosphorus, magnesium, calcium and barium, then either normal or ferrocene-loaded ferritin. Free-radical-mediated damage in the iron-loaded livers was inferred by the significant depletion of alpha-tocopherol in both the livers and subcellular hepatic lysosomal fraction, which inversely correlated with the increasing iron content (r = -0.61; P less than 0.05) and was associated with increased fragility of the lysosomal membranes.
给雄性Wistar大鼠喂食二茂铁6周后，其肝脏铁负荷较高（7.24±1.97毫克铁/克组织），主要位于溶酶体中，这与人类血色素沉着症中测定的水平和分布相当。从喂食二茂铁的大鼠肝脏中分离出两种铁储存蛋白，即铁蛋白和含铁血黄素，并通过穆斯堡尔光谱法和电感耦合等离子体发射光谱法研究了它们的铁芯。水铁矿是铁蛋白和含铁血黄素中铁的主要存在形式，而含铁血黄素中磷、镁、钙和钡的含量高于正常或喂食二茂铁的铁蛋白。肝脏和亚细胞肝溶酶体组分中α-生育酚的显著消耗表明铁负荷肝脏中存在自由基介导的损伤，这与铁含量增加呈负相关（r = -0.61；P<0.05），并与溶酶体膜的脆性增加有关。
