Ito H, Kaneko K, Makino R, Konishi K, Kurahashi T, Yamamoto T, Katagiri A, Kumekawa Y, Kubota Y, Muramoto T, Mitamura K, Imawari M
Second Department of Internal Medicine, Showa University School of Medicine, Tokyo 142-0064, Japan.
Oncol Rep. 2007 Aug;18(2):473-81.
Interleukin (IL)-1 gene polymorphisms are associated with development of gastric atrophy and with increased risk of gastric carcinoma. A -31C to T base transition in the promoter region of this gene is involved in carcinogenic changes within the stomach, especially in Helicobacter pylori infected individuals. We examined association between IL-1 locus polymorphisms and risk of esophageal, gastric and colorectal carcinomas in Japanese patients with H. pylori infection. IL-1B and IL-1RN polymorphisms were analyzed in 136 controls, 75 patients with esophageal carcinoma, 186 patients with gastric carcinoma, 69 patients with colorectal carcinoma, and 18 patients with ulcerative colitis (UC). For IL-1B-511 and -31 polymorphisms were determined by fluorescence-based polymerase chain reaction single-strand conformation polymorphism analysis. For IL-1 receptor antagonist gene (IL-1RN), penta-allelic variable number of tandem repeats (VNTR) was determined by PCR-standard agarose gel electrophoresis. For gastric carcinoma, IL-1B-511 heterozygotes (OR, 0.48; 95% CI, 0.3-0.9; p=0.0115) and T carriers (OR, 0.52; 95% CI, 0.3-1.0; p=0.0185) had a significantly reduced risk of carcinoma. For colorectal carcinoma, IL-1B-511 heterozygotes (OR, 0.34; 95% CI, 0.2-0.7; p=0.0028) and T carriers (OR, 0.43; 95% CI, 0.2-0.9; p=0.0015) had a significantly low risk of carcinoma. No significant difference was observed in the frequencies of IL-1B-31C/T and IL-1RN genotypes between controls and the esophageal carcinoma patients. Our results shows that IL-1B-511C/T and T carrier state may indicate less risk for gastric and colorectal carcinoma in the Japanese population.
白细胞介素（IL）-1基因多态性与胃萎缩的发生以及胃癌风险增加相关。该基因启动子区域的-31C到T碱基转换与胃内致癌变化有关，尤其是在幽门螺杆菌感染个体中。我们研究了日本幽门螺杆菌感染患者中IL-1基因座多态性与食管癌、胃癌和结直肠癌风险之间的关联。对136名对照者、75名食管癌患者、186名胃癌患者、69名结直肠癌患者和18名溃疡性结肠炎（UC）患者进行了IL-1B和IL-1RN多态性分析。对于IL-1B -511和-31多态性，通过基于荧光的聚合酶链反应单链构象多态性分析来确定。对于IL-1受体拮抗剂基因（IL-1RN），通过聚合酶链反应-标准琼脂糖凝胶电泳确定五等位基因串联重复序列（VNTR）的可变数目。对于胃癌，IL-1B -511杂合子（比值比[OR]，0.48；95%可信区间[CI]，0.3 - 0.9；p = 0.0115）和T等位基因携带者（OR，0.52；95% CI，0.3 - 1.0；p = 0.0185）患癌风险显著降低。对于结直肠癌，IL-1B -511杂合子（OR，0.34；95% CI，0.2 - 0.7；p = 0.0028）和T等位基因携带者（OR，0.43；95% CI，0.2 - 0.9；p = 0.0015）患癌风险显著较低。在对照者和食管癌患者之间未观察到IL-1B -31C/T和IL-1RN基因型频率的显著差异。我们的结果表明，在日本人群中，IL-1B -511C/T和T等位基因携带者状态可能表明患胃癌和结直肠癌的风险较低。
