Gahr Scott A, Rexroad Caird E, Rise Matthew L, Hunt Peter, Koop Ben
United States Department of Agriculture, Agriculture Research Service, National Center for Cool and Cold Water Aquaculture, Kearneysville, West Virginia 25430, USA.
Anim Biotechnol. 2007;18(3):213-30. doi: 10.1080/10495390701337335.
The pituitary plays significant roles in the regulation of physiological processes. In the current study, expressed sequence tag data was obtained for 1,920 clones from a normalized mixed-sex pituitary cDNA library. From these 3,840 sequences, a total of 524 contigs were assembled and 1,256 unique singletons identified. Assignment of functional annotation was performed through BLAST and gene ontology term assignment. Through in silico comparative mapping homologs were identified for 354 of the unigene sequences. These data provide the first functional information on many of the transcripts present in the rainbow trout pituitary.
垂体在生理过程的调节中发挥着重要作用。在本研究中，从一个标准化的混合性别的垂体cDNA文库中获得了1920个克隆的表达序列标签数据。从这3840个序列中，共组装了524个重叠群，并鉴定出1256个独特的单序列。通过BLAST和基因本体术语分配进行功能注释。通过电子比较图谱，为354个单基因序列鉴定出了同源物。这些数据为虹鳟鱼垂体中存在的许多转录本提供了首个功能信息。
