肌肉中质粒DNA体内转移的改进：电穿孔与超声的比较。

Improvement of in vivo transfer of plasmid DNA in muscle: comparison of electroporation versus ultrasound.

作者信息

Kusumanto Yoka H, Mulder Nanno H, Dam Wendy A, Losen Mario, De Baets Marc H, Meijer Coby, Hospers Geke A P

机构信息

Department of Medical Oncology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.

出版信息

Drug Deliv. 2007 Jul;14(5):273-7. doi: 10.1080/10717540601098807.

DOI:10.1080/10717540601098807

PMID:17613014

Abstract

Plasmid-based gene delivery to muscle is a treatment strategy for many diseases with potential advantages above viral-based gene delivery methods, however, with a relative low transfection efficiency. We compared two physical methods - electroporation and ultrasound - that facilitate DNA uptake into cells. Mice (C57Bl/6) were injected intramuscular using plasmid DNA encoding an intracellular protein (p53) followed by electroporation or ultrasound. Then 48 hr after the injections the mice were sacrificed. The parameter for transfection efficiency was the area of muscle expressing the transgene. The p53 expression plasmid showed a 36-fold increase (p = 0.015) in transfection efficiency with electroporation compared to ultrasound. Compared with ultrasound, electroporation significantly improves transfection efficiency of naked plasmid DNA transfer into skeletal muscle.

摘要

基于质粒的基因导入肌肉是许多疾病的一种治疗策略，与基于病毒的基因导入方法相比具有潜在优势，然而，其转染效率相对较低。我们比较了两种促进DNA进入细胞的物理方法——电穿孔和超声。将编码细胞内蛋白（p53）的质粒DNA经肌肉注射到小鼠（C57Bl/6）体内，随后进行电穿孔或超声处理。注射后48小时处死小鼠。转染效率的参数是表达转基因的肌肉面积。与超声相比，p53表达质粒经电穿孔处理后的转染效率提高了36倍（p = 0.015）。与超声相比，电穿孔显著提高了裸质粒DNA转入骨骼肌的转染效率。