Bodles-Brakhop Angela M, Heller Richard, Draghia-Akli Ruxandra
VGX Pharmaceuticals, Inc., 2700 Research Forest Drive, Suite 180, The Woodlands, Texas 77381, USA.
Mol Ther. 2009 Apr;17(4):585-92. doi: 10.1038/mt.2009.5. Epub 2009 Feb 17.
Electroporation (EP) has been used in basic research for the past 25 years to aid in the transfer of DNA into cells in vitro. EP in vivo enhances transfer of DNA vaccines and therapeutic plasmids to the skin, muscle, tumors, and other tissues resulting in high levels of expression, often with serological and clinical benefits. The recent interest in nonviral gene transfer as treatment options for a vast array of conditions has resulted in the refinement and optimization of EP technology. Current research has revealed that EP can be successfully used in many species, including humans. Clinical trials are currently under way. Herein, the transition of EP from basic science to clinical trials will be discussed.
在过去25年里,电穿孔(EP)已被用于基础研究,以协助将DNA体外导入细胞。体内电穿孔可增强DNA疫苗和治疗性质粒向皮肤、肌肉、肿瘤及其他组织的转移,从而实现高水平表达,且往往具有血清学和临床益处。近期,作为众多疾病治疗选择的非病毒基因转移备受关注,这促使电穿孔技术得到改进和优化。目前的研究表明,电穿孔可成功应用于包括人类在内的许多物种。目前临床试验正在进行中。本文将讨论电穿孔从基础科学向临床试验的转变。