Kawakami E, Tonsho H, Tsutsui T, Ogasa A
Department of Reproduction, Nippon Veterinary and Animal Science University, Japan.
Int J Androl. 1991 Dec;14(6):441-52. doi: 10.1111/j.1365-2605.1991.tb01273.x.
Three male dogs, aged 2-4 years, which exhibited a high percentage of sperm with abnormal tails in their semen and low levels of peripheral plasma LH and testosterone were given two injections of 400 micrograms an LHRH analogue (LHRH-A) 3 h apart. The semen quality of all three dogs and the peripheral plasma levels of LH and testosterone were examined in two of the dogs. Plasma levels of LH and testosterone in both dogs peaked at 30 and 90 min respectively, after the injection of LHRH-A. The semen quality of all three dogs had improved temporarily 20 days after the injection. To be specific, although the mean percentage of sperm with abnormal tails in the three dogs was 19, 26 and 32% respectively before injection of LHRH-A, these values had decreased to 10, 16 and 13%, respectively, 20-40 days later. Thereafter, however, the percentage sperm abnormalities increased again. The testes and epididymides of two of the dogs were examined histologically at the end of 90 days. Sloughed germ cells were observed in the seminiferous tubules of the testes, and pyknotic epithelial cells were noted in some of the epididymal ducts. The other dog, which had a history of three unsuccessful matings was treated again with LHRH-A and mated with three bitches 30, 75, and 110 days later, respectively. Only the bitch mated with this dog 30 days after a second series of injections conceived. It is assumed that the semen quality of the three dogs improved because of a temporary rise in plasma LH and testosterone levels after the injection of LHRH-A. It is concluded that the fertility of dogs with a high percentage of sperm with abnormal tails can be improved approximately 30 days after treatment with an LHRH-A.
三只年龄在2至4岁的雄性犬，其精液中尾部异常的精子比例较高，外周血浆促黄体生成素（LH）和睾酮水平较低，每隔3小时注射两次400微克促性腺激素释放激素类似物（LHRH-A）。对其中两只犬的精液质量以及外周血浆LH和睾酮水平进行了检测。注射LHRH-A后，两只犬的血浆LH和睾酮水平分别在30分钟和90分钟达到峰值。注射后20天，三只犬的精液质量均出现暂时改善。具体而言，在注射LHRH-A前，三只犬尾部异常精子的平均比例分别为19%、26%和32%，而在20至40天后，这些数值分别降至10%、16%和13%。然而，此后精子异常比例再次上升。在90天结束时，对其中两只犬的睾丸和附睾进行了组织学检查。在睾丸的生精小管中观察到脱落的生殖细胞，在一些附睾管中发现了固缩的上皮细胞。另一只犬有三次配种未成功的记录，再次用LHRH-A治疗，并分别在30天、75天和110天后与三只母犬交配。只有在第二次注射系列后30天与这只犬交配的母犬受孕。据推测，三只犬精液质量的改善是由于注射LHRH-A后血浆LH和睾酮水平暂时升高所致。得出结论，尾部异常精子比例较高的犬只在使用LHRH-A治疗后约30天，生育能力可得到改善。
