Liu Xiao, Chen Xiao-Hui, Zhang Yuan-Yuan, Liu Wen-Tao, Bi Kai-Shun
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 1;856(1-2):273-7. doi: 10.1016/j.jchromb.2007.06.009. Epub 2007 Jun 22.
A method based on cloud-point extraction (CPE) was developed to determine arbidol in rat plasma by high performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as the extract solvent. Variable parameters affecting the CPE efficiency were evaluated and optimized. A Zorbax SB-C(18) column (4.6 mm i.d. x 150 mm, 5 microm particle size) was used for isocratic elution separation at 40 degrees C with detection wavelength at 316 nm. Under the optimum conditions, the method was shown to be reproducible and reliable with intraday precision below 6.6%, interday precision below 8.8%, accuracy within +/-5.0% and mean extraction recovery more than 89.7%, which were all calculated using a range of spiked samples at three concentrations of 0.2, 2 and 16 microg/ml for arbidol in plasma. The linear range was from 0.08 to 20 microg/ml. After strict validation, the method was successfully applied to the pharmacokinetic study of arbidol in rats after oral and intravenous administration, respectively.
