Li Xiao, Wu Lingyun, Ying Shaoxu, Chang Chunkang, He Qi, Pu Quan
Department of Hematology, Shanghai Sixth Hospital, affiliated with Shanghai Jiao Tong University, Shanghai, China.
Leuk Lymphoma. 2007 Jul;48(7):1353-71. doi: 10.1080/10428190701395065.
Studies were performed to obtain evidence of the differentiating and hematopoietic potential of marrow clone-original cells in patients with myelodysplastic syndromes (MDS). First, results of correlation analysis between bone marrow clonal cells and blast percentages for a total of 60 MDS showed that almost all cases had higher clonal (mean 50.1%) than blast proportion (mean 7.0%) (p < 0.001). In contrast, in 16 AML patients, mean clone/blasts disparity was nearly zero. Secondly, the amount of clone-original individual cells were defined in mature hematopoietic cells, mean 47.9% in segmented granulocytes, 47.1% in orthochromatic normoblasts and 37.8% in mature megakaryocytes. In addition, FISH examination showed approximately similar proportions of clonal cells in peripheral blood and marrow for all 22 tested cases (mean 39.1% in blood vs 49.8% in marrow). Moreover, the neutrophils in MDS peripheral blood oxidized dihydrorhodamine123 (DHR) nearly the same as neutrophils in a normal donor's circulation, while obviously poor function was observed in neutrophils from AML blood. Of note, research on clonality had an unexpected outcome as most of the typical morphological dysplastic cells possessed normal karyotypes. These findings lead to the proposal that the biological features of MDS clones are distinctive from those of AML clones.
开展了多项研究，以获取骨髓增生异常综合征（MDS）患者骨髓克隆原始细胞的分化和造血潜能的证据。首先，对总共60例MDS患者的骨髓克隆细胞与原始细胞百分比进行相关性分析的结果显示，几乎所有病例的克隆比例（平均50.1%）均高于原始细胞比例（平均7.0%）（p<0.001）。相比之下，在16例急性髓系白血病（AML）患者中，克隆细胞与原始细胞的平均差异几乎为零。其次，在成熟造血细胞中确定了克隆原始单个细胞的数量，在分叶核粒细胞中平均为47.9%，在正染早幼粒细胞中为47.1%，在成熟巨核细胞中为37.8%。此外，荧光原位杂交（FISH）检查显示，在所有22例检测病例中，外周血和骨髓中的克隆细胞比例大致相似（外周血平均为39.1%，骨髓为49.8%）。此外，MDS外周血中的中性粒细胞氧化二氢罗丹明123（DHR）的情况与正常供体循环中的中性粒细胞几乎相同，而AML血液中的中性粒细胞功能明显较差。值得注意的是，克隆性研究有一个意外发现，即大多数典型形态发育异常的细胞具有正常核型。这些发现提示，MDS克隆的生物学特征与AML克隆不同。
