Li Xiao, Wu Ling-Yun, Ying Shao-Xu, Chang Chun-Kang, He Qi, Song Lu-Qian, Pu Quan
Department of Hematology, Sixth Peoples Hospital of Shanghai, Shanghai 200233, China.
Zhonghua Xue Ye Xue Za Zhi. 2007 Jul;28(7):478-83.
To investigate the biological difference of clonal cells between myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML).
Bone marrow (BM) clonal cells (which had cytogenetic markers detected by FISH assay) and blasts were quantitatively analysed in 51 MDS and 11 AML patients. The clonal cell percentage in orthochromatic normoblasts, granulocytes and megakaryocytes were assayed. The biological functions for phagocytosis and oxidation of MDS peripheral blood (PB) neutrophils were compared with that of normal controls.
Almost all MDS patients BM had a higher clonal cell percentage (mean 48.2%) than blasts percentage (mean 6.7%) (P < 0.01), but with the subtype of MDS advancing this percentage gap was closing up, and in 11 AML patients no such gap was observed. This gap in MDS patients with + 8 abnormality was smaller than in those with 5q -. In MDS BM, clonal cells were detected in segmented granulocytes (mean 45.9%), orthochromatic normoblasts (mean 46.0%) and mature megakaryocytes (mean 38.0%). In Addition, an approximate amount of clonal cells with the same karyotype abnormality in BM were detected in MDS PB (mean 37.3% in blood vs 48.6% in marrow). Functional analysis showed that the neutrophils in MDS PB could exert nearly normal physiological functions (P > 0.05), but those from AML could not as compared to healthy donors (P < 0.01).
There is a significant difference in the biological features between MDS and AML clonal cells.
探讨骨髓增生异常综合征（MDS）与急性髓系白血病（AML）克隆细胞的生物学差异。
对51例MDS患者和11例AML患者的骨髓（BM）克隆细胞（通过荧光原位杂交检测有细胞遗传学标记）和原始细胞进行定量分析。检测正染早幼粒细胞、粒细胞和巨核细胞中的克隆细胞百分比。将MDS外周血（PB）中性粒细胞的吞噬和氧化生物学功能与正常对照进行比较。
几乎所有MDS患者骨髓中的克隆细胞百分比（平均48.2%）高于原始细胞百分比（平均6.7%）（P<0.01），但随着MDS亚型进展，这一百分比差距在缩小，而11例AML患者未观察到这种差距。+8异常的MDS患者的这种差距小于5q-异常的患者。在MDS骨髓中，在分叶粒细胞（平均45.9%）、正染早幼粒细胞（平均46.0%）和成熟巨核细胞（平均38.0%）中检测到克隆细胞。此外，在MDS外周血中检测到与骨髓中具有相同核型异常的近似数量的克隆细胞（血液中平均37.3%，骨髓中48.6%）。功能分析表明，MDS外周血中的中性粒细胞可发挥近乎正常的生理功能（P>0.05），但与健康供体相比，AML患者的中性粒细胞则不能（P<0.01）。
MDS和AML克隆细胞的生物学特征存在显著差异。
